Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pectobacterium carotovorum strain:DSM 30169 | isolate:Brassica oleracea var. capitata, soft rot

ABSTRACT: Pectobacterium carotovorum strain:DSM 30169 | isolate:Brassica oleracea var. capitata, soft rot Genome sequencing

PROVIDER: PRJNA663365 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq of Pectobacterium carotovorum subsp. carotovorum and P. polaris infecting potato at 24, 48 and 72 hpi, and grown on M9 solid minimal medium at 24 hpi

Project description:Importance: Pectobacterium species cause soft rot in potato and other host plants primarily by secreting a battery of plant cell wall degrading enzymes. In addition, several different secretion systems are mobilized during infection. Previous studies of gene expression and regulation thereof primarily focused on the onset of infection. This work investigated transcriptome changes in Pectobacterium during the infection of potato tubers up to 72 hours post inoculation to elucidate biological processes during a longer infection period. Methods: The transcriptomes of aggressive strains of the two species P. carotovorum subsp. carotovorum and P. polaris were investigated during infection of potato minitubers (cv. 'Asterix') at 24, 48 and 72 hours after inoculation by RNA sequencing. The transcriptomes were compared to that of bacteria grown on minimal M9 medium, and transcriptomes from later infection time points (48 and 72 hours after inoculation) were compared to early infection (24 hours after inoculation). Results: Plant cell wall degrading enzymes and secretion system associated genes were largely upregulated during infection compared to in vitro growth, but downregulated in the later phases of infection compared to the early infection phase. The downregulation was not sufficiently explained by the expression of known virulence regulators such as the RsmA/B or the ExpA/S systems.

2022-04-01 | E-MTAB-7921 | biostudies-arrayexpress

Comparative transcriptome analysis reveals defense responses against soft rot in Chinese cabbage

Project description:Pectobacterium carotovorum ssp. carotovorum (Pcc) is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage. In this study, plants harboring the resistant mutant sr gene, which confers resistance against Pcc,were screened from an 800 M2 population mutated by ethyl methane sulfonate (EMS) and scored in vitro and in vivo for lesion size. The transcript profiles showed ~512 differentially expressed genes (DEGs) between sr and WT plants occurring between 6 and 12 h postinoculation (hpi), which corresponded to the important defense regulation period (resistance) to Pcc in Chinese cabbage. The downstream defense genes (CPK, CML, RBOH MPK3, and MPK4) of pathogen pattern-triggered immunity (PTI) were strongly activated during infection at 12 hpi in resistant mutant sr; PTI appears to be central to plant defense against Pcc via recognition by three putative pattern recognition receptors (PRRs; BrLYM1-BrCERK1, BrBKK1/SERK4-PEPR1, BrWAKs). Pcc triggered the upregulation of the jasmonic acid (JA) and ethylene (ET) biosynthesis genes in mutant sr, but auxins and other hormones may have affected some negative signals.Endogenous hormones (auxins, JAs, and SA), as well as exogenous auxins (MEJA and BTH), were also verified as functioning in the immune system. Concurrently, the expression of glucosinolate and lignin biosynthesis genes was increased at 12 hpi in resistant mutant sr, and the accumulation of glucosinolate and lignin also indicated that these genes have a functional defensive role against Pcc. Our study provides valuable information and elucidates the resistance mechanism of Chinese cabbage against Pcc infection.

2022-07-27 | GSE209906 | GEO

Transcription profiling of Nicotinan benthamiana in response to Pectobacterium carotovorum WPP14 and Pseudomonas syringae pv. tomato DC3000

Project description:Transcription profiling of Nicotinan benthamiana in response to Pectobacterium carotovorum WPP14 and Pseudomonas syringae pv. tomato DC3000

2010-07-24 | E-TABM-752 | biostudies-arrayexpress

Pectobacterium carotovorum subsp. carotovorum PCCS1

Project description:Pectobacterium carotovorum subsp. carotovorum Genome Sequencing

| PRJNA84409 | ENA

Pectobacterium carotovorum

Project description:Pectobacterium carotovorum overview

| PRJNA37001 | ENA

Pectobacterium carotovorum subsp. carotovorum PCC21

Project description:Pectobacterium carotovorum subsp. carotovorum PCC21 Genome sequencing

| PRJNA171866 | ENA

Pectobacterium carotovorum subsp. carotovorum PCCS1

Project description:Pectobacterium carotovorum subsp. carotovorum PCCS1 Genome sequencing