Project description:This is the first genome-scale model of G. dulcis, an extremely desiccation-tolerant cyanobacterium isolated from the Atacama desert by Azua-Bustos et al. (2014). We sequenced, closed, and annotated the G. dulcis genome, and created the model using KBase, and COBRApy. We included cyanobacterium-based photosynthesis reactions based on Toyoshima et al. (2020).
Project description:Native metabolomics and corresponding metabolomics data for new peptidic trypsin inhibitors isolated from the cyanobacterium Nostoc sp. KVJ20.
Project description:We isolated a cyanobacterium from H. splendens and co-incubated it with H. splendens shoots. Cyanobacterium and moss were also incubated separately. After one week, we performed acetylene reduction assays to estimate N2 fixation and RNAseq to evaluate transcriptomes.
Project description:Bryophytes comprise mosses, liverworts and hornworts. The chromatin landscapes of mosses and liverworts are different, leaving open the question regarding the identity of the chromatin landscape of all bryophytes. To address this question we obtained a genome wide profile of 5-methylated cytosine from a model hornwort, Anthoceros agrestis.
Project description:Previous molecular and mechanistic studies have identified several principles of prokaryotic transcription, but less is known about the global transcriptional architecture of bacterial genomes. Here we perform a comprehensive study of a cyanobacterial transcriptome, that of Synechococcus elongatus PCC 7942, generated by combining three high-resolution data sets: RNA sequencing, tiling expression microarrays, and RNA polymerase chromatin immunoprecipitation (ChIP) sequencing. We report absolute transcript levels, operon identification, and high-resolution mapping of 5' and 3' ends of transcripts. We identify several interesting features at promoters, within transcripts and in terminators relating to transcription initiation, elongation, and termination. Furthermore, we identify many putative non-coding transcripts. We provide a global analysis of a cyanobacterial transcriptome. Our results uncover insights that reinforce and extend the current views of bacterial transcription. RNA Sequencing of the cyanobacterium Synechococcus elongatus PCC 7942 RNA polymerase ChIP Sequencing of the cyanobacterium Synechococcus elongatus PCC 7942 Tiling Microarray of the cyanobacterium Synechococcus elongatus PCC 7942
Project description:Deciphering structure, function and mechanism of a new lysine methyltransferase cKMT1 in model cyanobacterium Synechocystis sp. PCC 6803