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Evolutionary Engineering of E. coli MG1655 for Tolerance Against Isoprenol

ABSTRACT: Evolutionary Engineering of E. coli MG1655 for Tolerance Against Isoprenol

PROVIDER: PRJNA667093 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12766333.fastq.gz	Fastqsanger.gz
	SRR12766334.fastq.gz	Fastqsanger.gz
	SRR12766335.fastq.gz	Fastqsanger.gz
	SRR12766336.fastq.gz	Fastqsanger.gz
	SRR12766337.fastq.gz	Fastqsanger.gz

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Evolutionary Engineering of E. coli MG1655 for Tolerance Against Isoprenol

Project description:Here we applied adaptive laboratory evolution successfully to evolve E. coli towards high tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. RNA-sequencing showed that the deletion identified upstream of yghB correlated with a strong overexpression of the gene.

2020-10-31 | GSE158959 | GEO

Evolutionary engineering of E. coli MG1655 for tolerance against isoprenol.

Project description:BackgroundIsoprenol is the basis for industrial flavor and vitamin synthesis and also a promising biofuel. Biotechnological production of isoprenol with E. coli is currently limited by the high toxicity of the final product. Adaptive laboratory evolution (ALE) is a promising method to address complex biological problems such as toxicity.ResultsHere we applied this method successfully to evolve E. coli towards higher tolerance against isoprenol, increasing growth at the half-maximal inhibitory concentration by 47%. Whole-genome re-sequencing of strains isolated from three replicate evolutions at seven time-points identified four major target genes for isoprenol tolerance: fabF, marC, yghB, and rob. We could show that knock-out of marC and expression of mutated Rob H(48) → frameshift increased tolerance against isoprenol and butanol. RNA-sequencing showed that the deletion identified upstream of yghB correlated with a strong overexpression of the gene. The knock-out of yghB demonstrated that it was essential for isoprenol tolerance. The mutated Rob protein and yghB deletion also lead to increased vanillin tolerance.ConclusionThrough ALE, novel targets for strain optimization in isoprenol production and also the production of other fuels, such as butanol, could be obtained. Their effectiveness could be shown through re-engineering. This paves the way for further optimization of E. coli for biofuel production.

| S-EPMC7653855 | biostudies-literature

Transcriptomic analysis of two ethanol-tolerant Saccharomyces cerevisiae clones obtained by evolutionary engineering

Project description:Evolutionary engineering strategy was used for selection of ethanol-tolerant Saccharomyces cerevisiae clones under gradually increasing ethanol stress levels. Clones B2 and B8 were selected based on their higher ethanol-tolerance and higher ethanol production levels. Whole genome microarray analysis was used for identifying the gene expression levels of these two evolved clones compared to the reference strain. Two evolved ethanol-tolerant strains B2 and B8, which were selected by evolutionary engineering under gradually increasing ethanol stress, were used for whole genome transcriptomic analysis in comparison with the reference strain. Cells were grown in yeast minimal media until they reach a final OD600 of 1. Following total RNA isolation, gene expression levels were analyzed using One-color microarray-based gene expression analysis (Agilent Technologies). Experiments were done in triplicates.

2016-03-01 | E-GEOD-78759 | biostudies-arrayexpress

Project description:Evolutionary Engineering of E. coli MG1655 for Tolerance Against Isoprenol

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Transcriptomic analysis of two ethanol-tolerant Saccharomyces cerevisiae clones obtained by evolutionary engineering

2016-03-01 | GSE78759 | GEO

Integrating metabolic and evolutionary engineering for enhanced propionic acid production under cross stress: a multi-omics perspective

Project description:We performed a combination of metabolic engineering (deletion of ldh and poxB and overexpression of mmc) with evolutionary engineering (selection under oxygen stress, acid stress and osmotic stress) in Propionibacterium acidipropionici. The results indicated that the mutants had superior physiological activity, especially the mutant III obtained from P. acidipropionici-Δldh-ΔpoxB+mmc by evolutionary engineering, with 1.5-3.5 times higher growth rates, as well as a 37.1% increase of PA titer and a 37.8% increase PA productivity compared to the wild type. Moreover, the 5.5-fold upregulation of Dps, 2 to 4-fold upregulation of ABC-type glycine betaine transporter and 3-fold upregulation of SOD indicated that these genes were likely in key regulons for the adaptation to abiotic stresses. An approximately 2.5-fold upregulation of mmc was also found. The results showed the multidirectional variation tendency of P. acidipropionici under cross stress and provide in-depth insights into the mechanism of tolerance and high production of PA.

2020-04-27 | PXD016616 | Pride

Evolutionary engineering of the pentose phosphate pathway in Saccharomyces cerevisiae.

Project description:Engineering the pentose phosphate pathway in Saccharomyces cerevisiae using an evolutionary engineering based strategy.

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Transcriptomic analysis of a caffeine-resistant Saccharomyces cerevisiae strain obtained by evolutionary engineering

Project description:A caffeine-resistant Saccharomyces cerevisiae mutant strain was obtained using an evolutionary engineering strategy based on successive batch cultivation at gradually increasing caffeine levels. The mutant strain Caf905-2 was selected at a caffeine concentration where its reference strain could not grow at all. Whole-genome transcriptomic analysis of Caf905-2 was performed with respect to its reference strain.

2018-12-28 | GSE124452 | GEO

Evolutionary Engineering and Transcriptomic Analysis of Phenylethanol-resistant Saccharomyces cerevisiae

Project description:Phenylethanol-resistant S. cerevisiae mutants were obtained by using evolutionary engineering strategy. Briefly, a chemically mutagenized culture was used as the initial population for the selection procedure. Gradually increasing levels of phenylethanol stress was applied through 56 successive batch cultivations. Individual mutants were selected from the final population. The mutant with the highest phenylethanol resistance could resist up to 3 g/L phenylethanol concentrations. Whole-genome transcriptomic analyses of the phenylethanol-resistant mutant strain and the reference strain were performed by using DNA microarray technology, in the absence of phenylethanol stress. Agilent yeast DNA microarray systems were used for whole-genome transcriptomic analyses of the reference strain and the selected phenylethanol-resistant mutant. Three replicates of each culture were grown in 100 mL yeast minimal medium using 500 mL flasks, at 30Â°C and 150 rpm. Cells at logarithmic phase of growth (~1.0 OD600) were used for total RNA isolation.

2014-07-12 | E-GEOD-59353 | biostudies-arrayexpress

Engineering chimeric PCSK9 for a vaccine against atherosclerosis

Project description:Engineering chimeric PCSK9 for a vaccine against atherosclerosis

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