Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen ATCC)

ABSTRACT: A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen ATCC)

PROVIDER: PRJNA670479 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12866200_1.fastq.gz	Fastqsanger.gz
	SRR12866200_2.fastq.gz	Fastqsanger.gz
	SRR12866300_1.fastq.gz	Fastqsanger.gz
	SRR12866300_2.fastq.gz	Fastqsanger.gz
	SRR12866400_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 576

Similar Datasets

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen PGP1)

Project description:A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen PGP1)

| PRJNA670566 | ENA

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen CRTD5)

Project description:A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen CRTD5)

| PRJNA670481 | ENA

Lentiviral shRNA library screen to identify growth-related genes in malignant melanoma

2014-01-24 | GSE46675 | GEO

Lentiviral shRNA library screen to identify growth-related genes in malignant melanoma

Project description:Little is known about genes that promote melanoma cell growth and proliferation. siRNAs may be used to address the role of individual genesin these processes. RNAi library screens were used in the past to gain a comprehensive overview of all genes involved in cell growth, proliferation, migration and other cellular processes. A large-scale loss-of-function screen for eight different melanoma cell lines was performed using a pooled lentiviral shRNA library (GeneNet Human 50K lentiviral shRNA Library,cat#SI206B-1, System Biosciences) to identify genes relevant for melanoma cell growth and proliferation. shRNAs that lead to cell death or reduced growth of transduced melanoma cells are negatively selected and thereby underrepresented in the final cellular shRNA pool and vice versa. The shRNAs of the shRNA library (3-5 per gene) have complementary sequences to probes on the custom Affymetrix microarray HG-U133Plus2 and were analysed using this array. Well-known melanoma cell lines SK-Mel-103, A375, SK-Mel-147, SK-Mel-19, SK-Mel-28, SK-Mel-29, SK-Mel-5, WM3523cln6 were transduced with the lentiviral shRNA library and grown for 10 days under puromycin selection (day 10), control cells of respective cell lines were transduced and frozen immediately after transduction and genomic integration of shRNAs (day 0). Totel DNA was extracted and genomically integrated shRNAs were hybridized to Affymetrix microarrays (HG-U133Plus2.0 array).

2014-01-24 | E-GEOD-46675 | biostudies-arrayexpress

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen ATCC)

Project description:Human pluripotent stem cell (hPSC)-derived cells offer an unprecedented opportunity to model diverse human tissue functions. However, the field lacks a generalized approach to rapidly and efficiently generate individual cell types of interest and to incorporate them into complex but defined tissues. To systematically explore the transcription factor (TF)-mediated hPSC programming landscape, we constructed the Human TFome: a comprehensive human TF library containing 1,564 TF (1,732 splice-isoforms). We discovered 241 previously unreported factors that individually converted hPSCs into diverse lineages with up to 99% efficiency in four days without alteration of external factors. Among these, we programmed neurons, fibroblasts, oligodendrocytes and endothelial cells that have molecular, transcriptomic and functional similarity to primary cells. Splice-isoform-specificity doubled endothelial conversion efficiency into in vivo-functional cells. Our cell-autonomous approach enabled parallel and orthogonal hPSCs programming into multiple cell types simultaneously without altering any microenvironmental cues. We generated in vivo-engraftable induced oligodendrocytes that expedited myelination within synthetically accelerated cerebral organoids.

2020-10-22 | GSE159779 | GEO

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen CRTD5)

2020-10-22 | GSE159781 | GEO

A comprehensive library of human transcription factors for cell fate engineering (Lentiviral Screen PGP1)

2020-10-22 | GSE159783 | GEO

A comprehensive library of human transcription factors for cell fate engineering

Project description:A comprehensive library of human transcription factors for cell fate engineering

| PRJNA670483 | ENA

Comprehensive Illumina Library Comparison

Project description:Comprehensive Illumina Library Comparison

| PRJEB31421 | ENA

A comprehensive library of human transcription factors for cell fate engineering (scRNA-Seq)