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ABSTRACT: WGS of swamp buffalo

PROVIDER: PRJNA670750 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR12915625_1.fastq.gz	Fastqsanger.gz
	SRR12915625_2.fastq.gz	Fastqsanger.gz
	SRR12915626_1.fastq.gz	Fastqsanger.gz
	SRR12915626_2.fastq.gz	Fastqsanger.gz
	SRR12915627_1.fastq.gz	Fastqsanger.gz

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Project description:Long non-coding RNAs (lncRNAs) have been identified in various tissues and cell types from human, monkey, porcine and mouse. However, expression profile of lncRNAs across Guangxi native cattle and swamp buffalo muscle development has never been investigated. Here, we examine the expression of lncRNA in cattle and buffalo muscle at adult stage(12 months), exhibiting the first report of lncRNA in the Guangxi native cattle and swamp buffalo muscle development of a large animal. 16,236 lncRNA candidates were obtained from buffalo skeletal muscle samples, of which a number of lncRNAs were highly abundant, and 2,161 lncRNAs were differentially expressed between buffalo and cattle. Real-time quantitative PCR (qPCR) analysis confirmed the expression profile of these lncRNAs, including several highly abundant lncRNAs, and a subset of differently expressed lncRNAs according to the high-throughput RNA sequencing (RNA-seq) data. These results indicate that abundant lncRNA is differentially expressed in bovine muscle, indicating important and diverse functions in mammalian muscle development.

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