Project description:Genome sequencing of UK duckweed panel including Lemna minor, Lemna japonica, Lemna minuta, Lemna turionifera and Spirodela polyrhiza accessions.
| PRJNA1026139 | ENA
Project description:Transcriptome sequencing of duckweed (Lemna turionifera)
Project description:Lemna minor a small aquatic plant has been used extensively in ecotoxicolgical testing to elucidate substance-related effects to freshwater plants. They are free-floating freshwater macrophyte, very sensitive towards chemical exposure and easy to cultivate thus makes the plant suitable for laboratory testing. Here we present a rapid and reproducible data dependent proteomics approach for identifying growth related molecular signatures in lemna minor as an alternative to algae testing. For this, we have analyzed the proteome of lemna minor exposed to bentazon as a model substances for identifying growth related molecular perturbations. These fingerprints allow for a definition of potential biomarkers as tools in screening approaches and for integration in plant growth inhibition studies, for identifying suspect substances, such as in the Lemna sp. growth inhibition test (OECD TG 221).
Project description:Duckweed-associated plant growth-promoting bacteria (PGPB) have attracted considerable attention for enhancing duckweed biomass production; however, the mechanisms underlying strain-specific plant growth promotion remain largely unknown. Here, we compared the effects of two duckweed-associated bacterial strains, Terrimicrobium sp. PS02 and Aeromicrobium sp. PS05, on the growth, biomass composition, colonization behavior, and transcriptomic responses of Spirodela polyrhiza. While both strains enhanced duckweed biomass, PS02 yielded a 1.2-fold increase, whereas PS05 induced a statistically significant increase (1.4-fold) compared with the uninoculated control. Markedly, PS05 established approximately one order of magnitude higher bacterial populations than PS02 and formed dense extracellular polymeric substance (EPS)-mediated microcolonies on duckweed surfaces. Transcriptome analysis revealed that PS05 induced 1,108 differentially expressed genes, compared with 454 genes for PS02, indicating substantially greater host transcriptomic reprogramming. Functional enrichment analyses demonstrated that PS05 preferentially regulated carbohydrate biosynthesis and central carbon metabolism. Consistently, both transcriptomic and RT-qPCR analyses showed up-regulation of key genes involved in starch biosynthesis and carbon allocation, resulting in significantly enhanced starch accumulation and turion formation without reducing protein or photosynthetic pigment content. This study provides the first integrated evidence linking bacterial colonization strategy, host transcriptomic regulation, carbon allocation, and biomass production in duckweed, thereby establishing a mechanistic basis for engineering high-performance duckweed–microbiome systems for sustainable biomass production.
Project description:Lemna minor a small aquatic plant has been used extensively in ecotoxicolgical testing to elucidate substance-related effects to freshwater plants. They are free-floating freshwater macrophyte, very sensitive towards chemical exposure and easy to cultivate thus makes the plant suitable for laboratory testing. Here we present a rapid and reproducible data dependent proteomics approach for identifying growth related molecular signatures in lemna minor as an alternative to algae testing. For this, we have analyzed the proteome of lemna minor exposed to atorvastatin as a model substances for identifying growth related molecular perturbations. These fingerprints allow for a definition of potential biomarkers as tools in screening approaches and for integration in plant growth inhibition studies (OECD TG 221), for identifying suspect substances.