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Identify Tfcp2l1-regulated genes in mouse embryonic stem cells grown in 2i medium

ABSTRACT: Identify Tfcp2l1-regulated genes in mouse embryonic stem cells grown in 2i medium

PROVIDER: PRJNA670915 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12884018_1.fastq.gz	Fastqsanger.gz
	SRR12884018_2.fastq.gz	Fastqsanger.gz
	SRR12884019_1.fastq.gz	Fastqsanger.gz
	SRR12884019_2.fastq.gz	Fastqsanger.gz
	SRR12884020_1.fastq.gz	Fastqsanger.gz

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Identify Tfcp2l1-regulated genes in mouse embryonic stem cells grown in 2i medium

Project description:Mouse embryonic stem cells (ESCs) grown in the 2i (an ERK inhibitor, PD0325901, and a GSK-3b inhibitor, CHIR99021) mimic pluripotent epiblasts in preimplantation blastocysts. ESCs grown in FBS containing medium are more heterogenous in morphology and expression of stem cell factors. Tfcp2l1 is a transcription factor highly expressed in ESCs grown in the 2i medium, suggesting that it plays in important role in ESC biology. To search for the putative role(s) of Tfcp2l1 in ESCs, we plan to use an integrated genomic approach--combing RNA-seq and ChIP-seq--to identify the direct targets of Tfcp2l1 in ESCs. RNA-seq is used to identify the transcripts regulated by Tfcp2l1 in ESCs grown in the 2i medium.

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Identify genomic binding sites of Tfcp2l1 in mouse embryonic stem cells grown in 2i medium

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