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The consequences of selection bias in analysis of pre-mRNA splicing

ABSTRACT: The consequences of selection bias in analysis of pre-mRNA splicing

PROVIDER: PRJNA671694 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12889359_1.fastq.gz	Fastqsanger.gz
	SRR12889359_2.fastq.gz	Fastqsanger.gz
	SRR12889360_1.fastq.gz	Fastqsanger.gz
	SRR12889360_2.fastq.gz	Fastqsanger.gz
	SRR12889361_1.fastq.gz	Fastqsanger.gz

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The consequences of selection bias in analysis of pre-mRNA splicing

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Project description:RNA splicing, the process of intron removal from pre-mRNA, is essential for the regulation of gene expression. It is controlled by the spliceosome, a megadalton RNA-protein complex that assembles de novo on each pre-mRNA intron via an ordered assembly of intermediate complexes. Spliceosome activation is a major control step requiring dramatic protein and RNA rearrangements leading to a catalytically active complex. Splicing factor 3B subunit 1 (SF3B1) protein, a subunit of the U2 snRNP, is phosphorylated during spliceosome activation, but the responsible kinase has not been identified. Here we show that cyclin-dependent kinase 11 (CDK11) associates with SF3B1 and phosphorylates threonine residues at its N-terminus during spliceosome activation. The phosphorylation is important for association of SF3B1 with U5 and U6 snRNAs in spliceosome activated Bact complex and it can be blocked by OTS964, a potent and selective inhibitor of CDK11. CDK11 inhibition prevents spliceosomal transition from the precatalytic complex B to the activated complex Bact and leads to widespread intron retention and accumulation of non-functional spliceosomes on pre-mRNAs and chromatin. We characterize OTS964 as a quality chemical biology probe for CDK11 and demonstrate a central role of CDK11 in spliceosome assembly and splicing regulation.

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