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I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells II

ABSTRACT: I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells II

PROVIDER: PRJNA672035 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12899139_1.fastq.gz	Fastqsanger.gz
	SRR12899139_2.fastq.gz	Fastqsanger.gz
	SRR12899140_1.fastq.gz	Fastqsanger.gz
	SRR12899140_2.fastq.gz	Fastqsanger.gz
	SRR12899141_1.fastq.gz	Fastqsanger.gz

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I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells

Project description:I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells

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I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells I

Project description:I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells I

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I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells II

2020-12-11 | GSE160126 | GEO

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I-KCKT allows dissection-free RNA profiling of adult <i>Drosophila</i> intestinal progenitor cells.

Project description:The adult Drosophila intestinal epithelium is a model system for stem cell biology, but its utility is limited by current biochemical methods that lack cell type resolution. Here, we describe a new proximity-based profiling method that relies upon a GAL4 driver, termed intestinal-kickout-GAL4 (I-KCKT-GAL4), that is exclusively expressed in intestinal progenitor cells. This method uses UV crosslinked whole animal frozen powder as its starting material to immunoprecipitate the RNA cargoes of transgenic epitope-tagged RNA binding proteins driven by I-KCKT-GAL4 When applied to the general mRNA-binder, poly(A)-binding protein, the RNA profile obtained by this method identifies 98.8% of transcripts found after progenitor cell sorting, and has low background noise despite being derived from whole animal lysate. We also mapped the targets of the more selective RNA binder, Fragile X mental retardation protein (FMRP), using enhanced crosslinking and immunoprecipitation (eCLIP), and report for the first time its binding motif in Drosophila cells. This method will therefore enable the RNA profiling of wild-type and mutant intestinal progenitor cells from intact flies exposed to normal and altered environments, as well as the identification of RNA-protein interactions crucial for stem cell function.

| S-EPMC7803463 | biostudies-literature

I-KCKT allows dissection-free RNA profiling of adult Drosophila intestinal progenitor cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-11 | GSE160128 | GEO

Functional Dissection of Transcription Factor Networks Governing Haematopoietic Progenitor States

Project description:Functional Dissection of Transcription Factor Networks Governing Haematopoietic Progenitor States

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aortic dissection molecular markers using iTRAQ combined with Label Free techniques

Project description:To screen serum molecular markers systematically amid aortic dissection and acute coronary syndrome, preliminary identify the pathogenesis of acute aortic dissection.

2022-03-25 | PXD032857 |

Functional dissection of lymphoid progenitor compartments resolves developmental trajectories in B-lymphocyte development

Project description:Functional dissection of lymphoid progenitor compartments resolves developmental trajectories in B-lymphocyte development

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Microarray analysis of in vitro differentiation of adult human pancreatic progenitor cells

Project description:We have developed an in vitro culture system that allows us to expand progenitor cells from human islet preparations and differentiate them into insulin-producing cells. We noticed however, that cultures from individual islet preparations had very heterogeneous outcomes, from good differentiation to almost none. We therefore speculated that our progenitor cell cultures contained different kinds of cells and that the true endocrine progenitor cells are present in the successful cultures, but not in the unsuccessful ones. To address this issue and to begin to identify markers for the true endocrine progenitor cells we compared global gene expression between a very successful culture (final insulin-expression 10% of islets) and an unsuccessful one (final insulin-expression 0%). We also included RNA from freshly isolated islets for control purposes. The cultures from donor A yielded substantial differentiation, while the cultures from donor B showed no successful differentiation.

2014-10-31 | E-MTAB-3050 | biostudies-arrayexpress

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