Project description:Campylobacter hepaticus, the causative agent of spotty liver disease (SLD) in commercial layer hens, leads to liver necrosis, reduced egg production, and increased mortality. Despite its impact, the pathogen’s virulence mechanisms remain poorly understood. This study used high-throughput transcriptomics to analyze gene expression profiles of C. hepaticus cultured in vitro bile, infected with LMH cells, and SLD affected chicken livers (in vivo) obtained from field cases to uncover key factors involved in host adaptation and pathogenicity
Project description:We are investigating the transcriptional response of mice infected with Helicobacter hepaticus and links to liver cancer We used microarrays to detail the global programme of gene expression underlying H. hepaticus induced liver cancer Keywords: disease model
Project description:Campylobacter jejuni is a common cause of diarrheal disease worldwide. Human infection typically occurs through the ingestion of contaminated poultry products. We previously demonstrated that an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan on its surface reduces the Campylobacter load in more than 50% of vaccinated leghorn and broiler birds to undetectable levels (responder birds), whereas the remainder of the animals were still colonized (non-responders). To understand the underlying mechanism, we conducted 3 larger scale vaccination and challenge studies using 135 broiler birds and found a similar responder/non responder effect. The submitted data were used for a genome-wide association study of the chicken responses to glycoconjugate vaccination against Campylobacter jejuni.
2021-11-01 | GSE181619 | GEO
Project description:Gut Microbiota of Spotty Liver Disease affected chickens
Project description:We evaluated aflatoxin B1-induced liver tumor promotion by H. hepaticus. Microarrays of liver and cecum from female mice were used to evaluate the individual and combined transcriptional effects of AFB1 and H. hepaticus Keywords: Tumor co-promotion study
Project description:We evaluated aflatoxin B1-induced liver tumor promotion by H. hepaticus. Microarrays of liver and cecum from female mice were used to evaluate the individual and combined transcriptional effects of AFB1 and H. hepaticus Experiment Overall Design: C3H/HeN mice were inoculated with 7 ug/g BW AFB1 or vehicle IP at 10 days of age, and gavaged with H. hepaticus or broth at 3 weeks; necropsied at 40 weeks