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Western and Heart Healthy Dietary Patterns Differentially Affect the Expression of Genes Associated with Lipid Metabolism, Interferon Signaling and Inflammation in the Jejunum of Ossabaw Pigs

ABSTRACT: Western and Heart Healthy Dietary Patterns Differentially Affect the Expression of Genes Associated with Lipid Metabolism, Interferon Signaling and Inflammation in the Jejunum of Ossabaw Pigs

PROVIDER: PRJNA673186 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12936512.fastq.gz	Fastqsanger.gz
	SRR12936513.fastq.gz	Fastqsanger.gz
	SRR12936514.fastq.gz	Fastqsanger.gz
	SRR12936515.fastq.gz	Fastqsanger.gz
	SRR12936516.fastq.gz	Fastqsanger.gz

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Western and Heart Healthy Dietary Patterns Differentially Affect the Expression of Genes Associated with Lipid Metabolism, Interferon Signaling and Inflammation in the Jejunum of Ossabaw Pigs

Project description:Overall diet quality and statin therapy are important modulators of inflammation and CAD progression, yet their effects on jejunum is not well understood. Our objective was to examine the effects and interaction of dietary patterns and statin therapy on jejunal mucosa gene expression in the Ossabaw pig.

2021-12-31 | GSE160426 | GEO

Western and heart healthy dietary patterns differentially affect the expression of genes associated with lipid metabolism, interferon signaling and inflammation in the jejunum of Ossabaw pigs.

Project description:Diet quality and statin therapy are established modulators of coronary artery disease (CAD) progression, but their effect on the gastrointestinal tract and subsequent sequelae that could affect CAD progression are relatively unexplored. To address this gap, Ossabaw pigs (N = 32) were randomly assigned to receive isocaloric amounts of a Western-type diet (WD; high in saturated fat, refined carbohydrate, and cholesterol, and low in fiber) or a heart healthy-type diet (HHD; high in unsaturated fat, whole grains, fruits and vegetables, supplemented with fish oil, and low in cholesterol), with or without atorvastatin, for 6 months. At the end of the study, RNA sequencing with 100 base pair single end reads on NextSeq 500 platform was conducted in isolated pig jejunal mucosa. A two-factor edgeR analysis revealed that the dietary patterns resulted in three differentially expressed genes related to lipid metabolism (SCD, FADS1, and SQLE). The expression of these genes was associated with cardiometabolic risk factors and atherosclerotic lesion severity. Subsequent gene enrichment analysis indicated the WD, compared to the HHD, resulted in higher interferon signaling and inflammation, with some of these genes being significantly associated with serum TNF-α and/or hsCRP concentrations, but not atherosclerotic lesion severity. No significant effect of atorvastatin therapy on gene expression, nor its interaction with dietary patterns, was identified. In conclusion, Western and heart healthy-type dietary patterns differentially affect the expression of genes associated with lipid metabolism, interferon signaling, and inflammation in the jejunum of Ossabaw pigs.

| S-EPMC8982565 | biostudies-literature

A Western Dietary Pattern and Atorvastatin Induce Epicardial Adipose Tissue Interferon Signaling in the Ossabaw Pig

Project description:Epicardial adipose tissue (EAT) inflammation is thought to potentiate the development of coronary artery disease (CAD). Overall diet quality and statin therapy are important modulators of inflammation and CAD progression. Our objective was to examine the effects and interaction of dietary patterns and statin therapy on EAT gene expression in the Ossabaw pig.

2020-02-04 | GSE129301 | GEO

A Western-type Dietary Pattern Induces an Atherogenic Gene Expression Profile in the Coronary Arteries of the Ossabaw Pig

Project description:We investigated the effect of 2 food-based dietary patterns and statin therapy on the transcriptome of the left anterior descending coronary artery of the Ossabaw pig.

2019-05-09 | GSE129300 | GEO

Colon Transcriptome is Modified by a Dietary Pattern/Atorvastatin Interaction in the Ossabaw Pig

Project description:Overall diet quality and statin therapy are important modulators of inflammation and CAD progression, yet their effects on colon is not well understood. Our objective was to examine the effects and interaction of dietary patterns and statin therapy on colonic mucosa gene expression in the Ossabaw pig.

2021-12-30 | GSE163159 | GEO

Differential regulation of brain microvessel transcriptome and brain metabolome by Western and heart-healthy dietary patterns in Ossabaw pig

Project description:Diet is a potentially modifiable neurodegenerative disease risk factor. We studied the effects of a typical Western diet (WD) relative to a heart-healthy diet (HHD) on microvessel transcriptomics and metabolomics of the brain temporal region in Ossabaw minipigs. Thirty-two pigs (16 male and 16 females) were randomized to the WD or HHD starting at the age of 4 months and were fed the assigned diet for the following 6 months. The WD and HHD were isocaloric and had the same macronutrient content but differed in macronutrient quality. Within each dietary group, half of the pigs also received a statin. Relative to HHD-fed pigs, WD-fed pigs had 2,172 genes differentially expressed (FDR<0.05) by diet, 796 upregulated and 1,376 downregulated. Gene Set Enrichment Analysis identified 22 gene sets enriched in WD, comprising pathways related to inflammation, angiogenesis, and apoptosis, and 53 gene sets enriched in the HHD, including cell energetics, neurotransmission, and inflammation resolution pathways. Enrichment in arginine, tyrosine, and lysine was observed in WD-fed pigs and enrichment in ergothioneine and S-adenosyl methionine in HHD-fed pigs. Statin treatment had very modest effects on brain vessel transcriptome. Our study suggests a likely contribution of diet to brain pathologies characterized by neuroinflammation and neurodegeneration.

2025-01-06 | GSE263935 | GEO

Differential regulation of brain microvessel transcriptome and brain metabolome by western and heart-healthy dietary patterns in Ossabaw pigs.

Project description:Diet is a potentially modifiable neurodegenerative disease risk factor. We studied the effects of a typical Western diet (WD; high in refined carbohydrates, cholesterol and saturated fat), relative to a heart-healthy diet (HHD; high in unrefined carbohydrates, polyunsaturated fat and fiber, and low in cholesterol) on brain microvessel transcriptomics and brain metabolomics of the temporal region in Ossabaw minipigs. Thirty-two pigs (16 male and 16 females) were fed a WD or HHD starting at the age of 4 months for a period of 6 months. The WD and HHD were isocaloric and had a similar macronutrient content but differed in macronutrient quality. Within each dietary group, half of the pigs also received atorvastatin. Relative to HHD-fed pigs, WD-fed pigs had 175 genes differentially expressed (fold change > 1.3, FDR < 0.05) by diet, 46 upregulated and 129 downregulated. Gene Set Enrichment Analysis identified 22 gene sets enriched in WD-fed pigs, comprising pathways related to inflammation, angiogenesis, and apoptosis, and 53 gene sets enriched in the HHD-fed pigs, including cell energetics, neurotransmission, and inflammation resolution pathways. Metabolite analysis showed enrichment in arginine, tyrosine, and lysine in WD-fed pigs, and ergothioneine and S-adenosyl methionine in HHD-fed pigs. Atorvastatin treatment did not affect gene expression. These results suggest a likely contribution of diet to brain pathologies characterized by neuroinflammation and neurodegeneration.

| S-EPMC11604918 | biostudies-literature

A Western Dietary Pattern and Atorvastatin Induce Epicardial Adipose Tissue Interferon Signaling in the Ossabaw Pig

Project description:A Western Dietary Pattern and Atorvastatin Induce Epicardial Adipose Tissue Interferon Signaling in the Ossabaw Pig

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Single nuclei RNA-seq of Ossabaw pig atherosclerotic and healthy aortae

Project description:Atherosclerosis is a major contributor to cardiovascular diseases. Well-established preclinical models that can accurately recapitulate human atherosclerosis development and progression are necessary for developing new therapeutics. Pig models are extensively used in cardiovascular research due to their physiological similarities to humans. However, their translational efficacy into clinical settings has remained limited. To ensure better translational outcomes, a deeper molecular characterization of atherosclerotic disease mechanisms in swine models is urgently required. Methods: To address this gap, we employed single nuclei transcriptomics to characterize healthy and atherosclerotic aortas in the Ossabaw pig model. We analyzed over 36,000 nuclei from aortas of 5 adult male castrated pigs kept on atherogenic and chow diets. In-depth single-nuclei transcriptomic and cell-cell communication analyses were undertaken to uncover molecular changes in swine atherosclerosis development. Comparisons to human and mouse atherosclerosis were used to establish the veracity of the Ossabaw pig in modeling human disease.

2025-08-10 | GSE274218 | GEO

RNA sequencing of Jejunum of WT and IL23 expression mice (KSR23)

Project description:The goal of this study is to check if elevated level of IL-23 and/or its downstream cytokines affect the gene expression in the jejunum of newborn mice. We engineered a mice strain in which the expression of IL-23 was directed to the keratinocytes (KSR23). The KSR23 mice had normal body weight at birth and much smaller than their control littermates at day 5. KSR23 mice died prematurely (before 15 days of age), but displayed no signs of disease in the skin, intestine, or in other organs examined. To investigate if elevated level of IL-23 and/or its downstreamcytokines could affect body growth, we examined the transcriptome of the the intestine, which are critical for the processing and absorption of nutrients. Jejunum mRNA profiles of 5-day-old WT and KSR23 were generated by deep sequencing. The transcriptome of the Jejunum of KSR23 mice at P5 differed significantly from that of their control littermates (WT). Several genes were differentially expressed, including IL-22, and genes that are downstream of it such as Reg3 genes and digestive enzymes also produced by the jejunum. Kegg pathway analysis of WT and KSR23 jejunum indicated that genes involved in protein digestion and absorption such as chymotrypsinogen B1 (Ctrb1), trypsinogen (Prss1), trypsin (Tyr5 and Tyr4), caboxypeptidase a1 (Cpa1) and chymotrypsin-like elastase family member 3b (Cela3b), were downregulated in the intestine of KSR23 mice. Transcriptome analysis also showed decreased expression of several genes involved in the regulation of the very low-density lipoprotein particle pathway (VLDL). These particles regulate fat and cholesterol release into the bloodstream. Together, these results indicate that systemic expression of IL-23, and other cytokines, correlated with significant transcriptional changes in genes that regulate food processing in the intestine.

2019-09-14 | GSE137413 | GEO

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