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Transcriptomic analysis of ETV2-overexpressing human dental pulp stem cells

ABSTRACT: Transcriptomic analysis of ETV2-overexpressing human dental pulp stem cells

PROVIDER: PRJNA673219 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12937944_1.fastq.gz	Fastqsanger.gz
	SRR12937944_2.fastq.gz	Fastqsanger.gz
	SRR12937945_1.fastq.gz	Fastqsanger.gz
	SRR12937945_2.fastq.gz	Fastqsanger.gz
	SRR12937946_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of ETV2-overexpressing human dental pulp stem cells

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The proteomics analysis of ETV2-overexpressing dental pulp stem cells

Project description:DPSCs derivedgenerated from the three different donors were transfectedinfected with ETV2-overexpressing lentiviralrus particles or control lentiviralus particles. Protein samples were harvested after 10 days of endothelial differentiation. Proteome profiling was performed by the iProteome platform of the Fudan University Human Phenome Institute according to user guide.

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Development of gene expression signatures for SOX2 overexpressing human dental pulp stem cells

Project description:As a key factor for cell pluripotent and self-renewing phenotypes, SOX2 has attracted scientists’ attention gradually in recent years. However, its exact effects in dental pulp stem cells (DPSCs) are still unclear. In this study, we mainly investigated whether SOX2 could affect some biological functions of DPSCs.

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Project description:Stem cell-based therapy is an alternative strategy for brain repair. Various cell types have been investigated and dental pulp stem cells (DPSC) have been identified as promising candidates. These multipotent stem cells are found in the dental pulp tissue of molar teeth. They are clinically easily obtained, have a high proliferative rate, and possess neurogenic potential due to their mesoectodermal origin. Here, overexpression of octamer-binding transcription factor 4 (OCT4) in combination with neural conditions was used to reprogram human DPSC along the neural lineage. Transcriptomic analysis of differentially-expressed genes highlighted the expression of genes associated with neural and neuronal functions in the OCT4-overexpressing DPSC following neural induction.

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Differential gene expression of Wnt ligands/Wnt agonist treated human dental pulp stem cells

Project description:Wnt regulates various cell responses. In dental pulp cells, Wnt signaling control cell proliferation, apoptosis, migration and differentiation. Here, the differential gene expression of human dental pulp stem cells treated with Wnt ligands or Wnt agonist was examined using a high throughput RNA sequencing technique. Results demonstrated that Wnt ligands or Wnt agonist altered numerous gene expression in human dental pulp stem cells.

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Chemotactic Signalling Triggers Dental Pulp Fibrosis in the Stem Cell Niches During

Project description:Aging often triggers dental pulp fibrosis, leading to various clinical repercussions, including heightened vulnerability to dental infections, compromised tooth vitality, and reduced responsiveness to dental interventions. Despite its common occurrence in clinical settings, the precise molecular mechanisms driving this condition remain elusive. Leveraging single-cell transcriptome analysis from both our own and publicly available datasets, we identified Ccrl2+ macrophages as particularly prone to susceptibility during the early stages of aging. Notably, dental pulp progenitors exhibiting high expression of Rarres2, a unique ligand for Ccrl2, orchestrate the selective recruitment of a specific macrophage population to the stem cell niches. Subsequently, this results in the presentation of the ligand-receptor complex to Cmklr1, a receptor ubiquitously expressed among all macrophage populations, ultimately leading to macrophage activation and expansion via the Rarres2/Ccrl2/Cmklr1 axis. Our study, supported by rigorous experimental validation, conclusively shows that macrophage activation and expansion in stem cell niches lead to increased secretion of proinflammatory factors, which contribute to dental pulp fibrosis during aging. Our data reveals the complex molecular dynamics of dental pulp aging, particularly focusing on interactions within the immune microenvironment. It offers a novel perspective on potential treatments for age-related pulp diseases, highlighting the role of macrophages and the possibility of modifying the immune microenvironment for therapeutic benefit.

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