Project description:In this study, 3,869 donkey skeletal muscle lncRNAs were identified using RNA-Seq along with a stringent screening procedure in the longissimus dorsi (LD) and gluteal (G) muscles. These lncRNAs share many characteristics with other mammalian lncRNAs, such as shorter open reading frames (ORFs) and lower expression levels than mRNAs. Furthermore, in pairwise comparisons between libraries of the same stage for two genetic types of male Dezhou donkey, 73 differentially expressed lncRNAs were common to all muscle tissues.
Project description:(1) Introduction: The ovaries are the reproductive organs of female animals and play a key role in fertility and maintaining endocrine balance. Gonadal development is a complex process that involves sex determination and subsequent maturation into the ovaries or testes. At present, the understanding of the mechanisms of donkey gonad development is still limited. (2) Objectives: This study explores the comprehensive single-cell transcriptomics of donkey gonads, providing insights into the cellular and molecular events behind sex norms and gonad differentiation. (3) Methods: We used single-cell sequencing technology to construct a high-resolution map of fetal donkey gonad development, analyze the diversity and complexity of the cell composition of donkey gonads at different developmental stages, and reveal the characteristics of key cell types and gene expression patterns. (4) Results: The different cell states and active molecular pathways during the development of donkey gonadal tissue were revealed, and the gonadal ridge developed into ovary at 4-8 weeks. (5) Conclusions: This study provides a foundational resource for understanding the complex biology of donkey gonadal development and may have implications for donkey reproductive health and fertility.
2026-07-01 | GSE290288 | GEO
Project description:The bacterial diversity in feces of Dezhou donkey
Project description:With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.In this study, we constructed a single-cell RNA dataset to study the single-cell landscape of donkey spermatogenesis and maturation. This method allows us to analyze the cell composition in testicular and epididymal tissue, providing insights into the changes that occur during donkey spermatogenesis and maturation. In addition, different gene expression signatures associated with various spermatogenic cell types were found