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ABSTRACT: intestinal Microbial diversity of Weaned piglets

PROVIDER: PRJNA677843 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13068413.fastq.gz	Fastqsanger.gz
	SRR13068414.fastq.gz	Fastqsanger.gz
	SRR13068415.fastq.gz	Fastqsanger.gz
	SRR13068416.fastq.gz	Fastqsanger.gz
	SRR13068417.fastq.gz	Fastqsanger.gz

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Project description:Intestinal microbial diversity on weaned piglets

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Transcription profiling of piglet ileal tissues to assess the impact of environment on microbial diversity and global transcriptional responses in the developing gut

Project description:The study investigated the impact of environment on the composition of the gut microbiota and mucosal immune development and function at gut surfaces in early and adult life. Piglets of similar genotype were reared in indoor and outdoor environments and in an experimental isolator facility. Mucosa-adherent microbial diversity in the pig ileum was characterized by sequence analysis of 16S rRNA gene libraries. Host-specific gene responses in gut ileal tissues to differences in microbial composition were investigated using Affymetrix microarray technology and Real-time PCR. Experiment Overall Design: Animals were reared on the sow at an outdoor or indoor facility. Additional piglets from the indoor facility were transferred to individual isolator units at 24 hours of age, and given a daily dose of antibiotic cocktail for the duration of the study. Piglets were weaned at day 28. From day 29 onwards, piglets were fed creep feed ad libitum. Ileal tissue samples were excised from N=6 piglets per group at day 5, 28 and 56.

2009-12-05 | E-GEOD-15256 | biostudies-arrayexpress

Project description:Studies of intestinal microbial diversity on weaned piglets

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Suckling piglets: Control vs. weaned piglets

Project description:Transcriptional profiling of 25d old piglets comparing control untreated suckling jejunum with weaned piglets' jejunum. The goal was to gain new insight into the interaction between weaning and intestinal function.A keen interest is paid in deciphering expression changes of apoptosis or cell cycle control genes. The statistical analysis of gene ontology revealed that most of these altered genes are metabolic-related enzymes and regulators which may involved in the biological regulation, developmental process, and cellular process. Weaning also causes alterations in various immune response pathways. Results likely indicate that weaning induced cell cycle arrest, enhanced apoptosis, and inhibited cell proliferation. Two-condition experiment, suckling control piglets' jejunum vs. weaned piglets' jejunum. Biological replicates: 4 control replicates, 4 weaned replicates.

2014-05-01 | E-GEOD-48050 | biostudies-arrayexpress

Pig Feces Metaproteome, LC-MSMS, TMT12Plex

Project description:Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a TMT LC-MSMS metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein abundances as they pertain to microbial populations specific to either a milk- or plant-based diet. Fecal samples were collected from six piglets on the day of weaning and four weeks after transitioning to a standard nursery diet. Using the 12,554 protein groups identified in samples, we confirmed the shift in protein composition that takes place in response to the microbial succession following weaning and demonstrated the redundancy in metabolic processes between taxa. We identified taxa with roles as primary degraders based on corresponding proteins synthesized, thereby providing evidence for cross-feeding. Proteins associated with the breakdown of milk-specific carbohydrates were common among pre-weaned pigs, whereas the proteome of post-weaned piglets contained a greater abundance of proteins involved in the breaking down plant-specific carbohydrates. Furthermore, output revealed that production of propionate takes place via the propionaldehyde pathway in pre-weaned piglets, but changes to production via the succinate pathway in post-weaned piglets. Finally, a disproportionate quantity of carbohydrate-active enzymes (CAZymes) (~8%) were produced by fungi, which typically only represent ~0.1% of the microbiome taxa. Information gathered through this characterization of the metaproteome before and after weaning revealed important differences regarding the role of members in the microbial community, thereby providing information for the optimization of diets and products for both piglet and microbiome health.

2025-05-26 | PXD063155 | Pride

Project description:Microbial diversity on weaned piglets

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Project description:Intestinal microbial community of weaned piglets

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The effect of dietary Spirulina microalga on the intestinal function of the post-weaned piglet

Project description:The aim of this study was to characterize the metabolomic and proteomic changes in the intestinal tissue of weaned piglets fed with diets containing Spirulina as an ingredient (10% incorporation in the diet), combining such information with histomorphology data.

2022-09-20 | PXD030446 | Pride

Effects of short distance transportation on physiological indexes, intestinal morphology, microbial community and transcriptome of jejunum in Weaned Piglets

Project description:To investigate the effect of short distance transport on jejunal tissueof weaned piglets, We then performed gene expression profiling analysis using data obtained from RNA-seq in jejunal tissues of weaned piglets after transport and without transport

2023-02-17 | GSE225156 | GEO

Project description:Studies of microbial diversity on weaned piglets.

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