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A role for condensin in mediating transcriptional adaptation to environmental stimuli. [RNA-Seq]

ABSTRACT: A role for condensin in mediating transcriptional adaptation to environmental stimuli. [RNA-Seq]

PROVIDER: PRJNA678750 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13068900.fastq.gz	Fastqsanger.gz
	SRR13068901.fastq.gz	Fastqsanger.gz
	SRR13068902.fastq.gz	Fastqsanger.gz
	SRR13068903.fastq.gz	Fastqsanger.gz
	SRR13068904.fastq.gz	Fastqsanger.gz

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A role for condensin in mediating transcriptional adaptation to environmental stimuli.

Project description:A role for condensin in mediating transcriptional adaptation to environmental stimuli.

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A role for condensin in mediating transcriptional adaptation to environmental stimuli. [TT-Seq]

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A role for condensin in mediating transcriptional adaptation to environmental stimuli. [TT-Seq]

Project description:The relationship between chromatin and transcription was investigated through condensin, a key regulator of higher order chromatin structure. We use rapid conditional condensin depletion in nocodozole arrested cells in budding yeast S. cerevisiae cells to study condensin’s role in transcriptional regulation. We use TT-Chemseq to assess global RNA levels through a S. pombe spike-in, and found that condensin depletion leads to upregulation of global transcription. We also found transcriptional changes in response to environmental stimuli are subdued by condensin depletion.

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A role for condensin in mediating transcriptional adaptation to environmental stimuli.

Project description:Nuclear organisation shapes gene regulation; however, the principles by which three-dimensional genome architecture influences gene transcription are incompletely understood. Condensin is a key architectural chromatin constituent, best known for its role in mitotic chromosome condensation. Yet at least a subset of condensin is bound to DNA throughout the cell cycle. Studies in various organisms have reported roles for condensin in transcriptional regulation, but no unifying mechanism has emerged. Here, we use rapid conditional condensin depletion in the budding yeast Saccharomyces cerevisiae to study its role in transcriptional regulation. We observe a large number of small gene expression changes, enriched at genes located close to condensin-binding sites, consistent with a possible local effect of condensin on gene expression. Furthermore, nascent RNA sequencing reveals that transcriptional down-regulation in response to environmental stimuli, in particular to heat shock, is subdued without condensin. Our results underscore the multitude by which an architectural chromosome constituent can affect gene regulation and suggest that condensin facilitates transcriptional reprogramming as part of adaptation to environmental changes.

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A role for condensin in mediating transcriptional adaptation to environmental stimuli.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Condensin II complex mediates interactions of histone gene loci

Project description:Condensin complexes are known for their importance in chromosome condensation during mitosis. However, condensin II binds to the geome during interphase as well. The role of condensin II in genome organization in mammalian interphase nuclei is not characterized. Since condensin II binds to the promoters of the transcriptionally active promoters in interphase genome, the aim of this study is to uncover its role in mediating chromatin interactions between active gene promoters such as histone gene loci.

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