Project description:Once ovulated, the oocyte has to be fertilized in a short time window, or it will undergo post-ovulation aging (POA), whose underlying mechanisms are still not elucidated. Here, we optimized single-cell proteomics methods and performed single-cell proteomic and phosphoproteomic analysis of fresh, POA, melatonin-treated and MG132-treated POA oocytes.
Project description:PN2 male and female rat pups were treated with vehicle or zebularine. POA RNA was extracted and sequenced to deterime the effects of DNMT inhibition in POA gene expression. Males and Females were treated with either vehicle (0.1% DNMSO in saline) or Zebularine