Project description:Here we used activating (purmorphamine) and blocking (cyclopamine) drugs characterized of the Hedgehog pathway in adipose tissue-derived stem cells and identified mRNAs associated polysomes or free fraction in each condition.
Project description:Low-coverage whole genome sequencing of cell-free DNA from immunosuppressed cancer patients enables tumor fraction determination and reveals relevant copy number alterations
Project description:To evaluate the efficiency of apatinib on vascularized patient-derived tumor organoids (PDTOs)-on-a-chip derived from patients with metastatic tumors, we conducted RNA-seq analysis on organoids derived from patients whom had metastatic tumors. Specifically, we extracted RNA for sequencing from tumor cells of organoids either cultured alone or co-cultured with MVs with or without apatinib treatment
Project description:Breast cancers of the luminal B subtype are frequent tumors with high proliferation and poor prognosis. Epigenetic alterations have been found in breast tumors and in biological fluids. We aimed to profile the cell-free DNA (cfDNA) methylome of metastatic luminal B breast cancer (LBBC) patients using an epigenomic approach to discover potential noninvasive biomarkers. Plasma cfDNA was analyzed using the Infinium MethylationEpic array (EPIC array) in a cohort of 14 women, including metastatic LBBC patients and healthy controls.
Project description:Because of limited clinical cases, the natural history of occult breast cancer (OBC) is poorly understood and its proteomic signature remains unknown. We performed a quantitative proteomic analysis for tissue samples of metastatic lymph nodes from 3 OBC patients, and paired tissue samples of metastatic lymph nodes and primary tumor from 3 Non-OBC patients.