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Next Generation Sequencing of Transcriptomes in SKOV3 and rhCCL20-treated SKOV3 cells

ABSTRACT: Next Generation Sequencing of Transcriptomes in SKOV3 and rhCCL20-treated SKOV3 cells

PROVIDER: PRJNA679482 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13084900_1.fastq.gz	Fastqsanger.gz
	SRR13084900_2.fastq.gz	Fastqsanger.gz
	SRR13084901_1.fastq.gz	Fastqsanger.gz
	SRR13084901_2.fastq.gz	Fastqsanger.gz
	SRR13084902_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing of Transcriptomes in SKOV3 and rhCCL20-treated SKOV3 cells

Project description:The goals of this study are to compare the transcriptome differences between SKOV3 and rhCCL20-treated SKOV3 cells and identify the defferential expressed genes regulated by rhCCL20 in SKOV3 cells. We treated SKOV3 cells with 5% trehalose (control group) and rhCCL20 protein dissolved in 5% trehalose (experimental group) in vitro. The cells were collected 24 hours after treatment and used for RNA-sequencing.

2020-11-20 | GSE161799 | GEO

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Project description:Purpose: Next generation sequencing (NGS) has revolutionized system-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling of human ovarian cancer cell, SKOV3 cells after endogenous overexpression of TNFAIP8L2 (TIPE2) . Methods: We constructed TIPE2 overexpression SKOV3 cell lines and control vector cell lines with lentivirus. Then the NGS based deep sequencing analysis was generated in triplicate to analyze the critical downstream targets after TIPE2 overexpression using ILLumina GAIIx. Results: The differentially expressed genes were identified using an optimized data analysis workflow, and we identified 49 upregulated and 28 downregulated genes in the SKOV3/TIPE2 group compared with the vector group using a |log2Foldchange|﹥0 and p < 0.05. Conclusions: Our study represents the first detailed analysis of transcriptomes in ovarian cancer cell line after overexpression of TIPE2 by RNA-seq technology.

2022-07-21 | GSE208351 | GEO

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