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Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression

ABSTRACT: Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression

PROVIDER: PRJNA680732 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13132878.fastq.gz	Fastqsanger.gz
	SRR13132879.fastq.gz	Fastqsanger.gz
	SRR13132880.fastq.gz	Fastqsanger.gz
	SRR13132881.fastq.gz	Fastqsanger.gz
	SRR13132882.fastq.gz	Fastqsanger.gz

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Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression [smallRNA-seq]

Project description:First-degree relatives (FDRs) of type 2 diabetics (T2D) feature dysfunction of subcutaneous adipose tissue (SAT) long before T2D onset. miRNAs have a role in adipocyte precursor cells (APC) differentiation and in adipocyte identity. Thus, impaired miRNA expression may contribute to SAT dysfunction in FDRs. In the present work, we have explored changes of miRNA expression associated with T2D family history which may affect gene expression in SAT APCs from FDRs. Small RNA-seq was performed in APCs from healthy FDRs and matched controls and omics data were validated by qPCR. Integrative analyses of APC miRNome and transcriptome from FDRs revealed down-regulated hsa-miR-23a-5p, -193a-5p and -193b-5p accompanied by up-regulated Insulin-like Growth Factor 2 (IGF2) gene which proved to be their direct target. The expression changes of these marks were associated with SAT adipocyte hypertrophy in FDRs. APCs from FDRs further demonstrated reduced capability to differentiate into adipocytes. Treatment with IGF2 protein decreased APC adipogenesis, while over-expression of hsa-miR-23a-5p, -193a-5p and -193b-5p enhanced adipogenesis by IGF2 targeting. Indeed, IGF2 increased the Wnt Family Member 10B gene expression in APCs. Down-regulation of the three miRNAs and IGF2 up-regulation were also observed in Peripheral Blood Leukocytes (PBLs) from FDRs. In conclusion, APCs from FDRs feature a specific miRNA/gene profile, which associates with SAT adipocyte hypertrophy and appears to contribute to impaired adipogenesis. PBL detection of this profile may help in identifying adipocyte hypertrophy in individuals at high risk of T2D.

2021-11-19 | GSE162133 | GEO

Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression [RNA-Seq]

2021-11-19 | GSE161989 | GEO

Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression [smallRNA-seq]

Project description:Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression [smallRNA-seq]

| PRJNA680747 | ENA

Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression [RNA-Seq]

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Increased serum miR-193a-5p during non-alcoholic fatty liver disease progression: diagnostic and mechanistic relevance

Project description:Background & Aims: Serum microRNAs (miRNAs) levels are known to change in non-alcoholic fatty liver disease (NAFLD) and may serve as useful biomarkers. This study aimed to profile miRNAs comprehensively at all NAFLD stages. Methods:We profiled 2,083 serum miRNAs in a discovery cohort (183 NAFLD cases representing the complete NAFLD spectrum and 10 population controls). MiRNA libraries generated by HTG EdgeSeq were sequenced by Illumina NextSeq. Selected serum miRNAs were profiled in 372 additional NAFLD cases and 15 population controls by quantitative reverse transcriptase-polymerase chain reaction. Results: Levels of 275 miRNAs differed between cases and population controls. Fewer differences were seen within individual NAFLD stages but miR-193a-5p consistently the showed increased levels in all comparisons. Relative to NAFL/NASH with mild fibrosis (stage 0/1), three miRNAs (miR-193a-5p, miR-378d and miR378d) were increased in cases with NASH and clinically significant fibrosis (stage 2-4), seven (miR193a-5p, miR-378d, miR-378e, miR-320b, c, d & e) increased in cases with NAFLD Activity Score (NAS) 5-8 compared with lower NAS, and three (miR-193a-5p, miR-378d, miR-378e) increased but one (miR-19b-3p) decreased in steatosis, activity, and fibrosis "activity" (SAF-A) score 2-4 compared with lower SAF-A. The significant findings for miR-193a-5p were replicated in the additional NAFLD cohort. Studies in Hep G2 cells showed that following palmitic acid treatment, miR-193a-5p expression decreased significantly. Gene targets for miR-193a-5p were investigated in liver RNAseq data for a case subgroup (n=80); liver GPX8 levels correlated positively with serum miR-193a-5p. Conclusions: Serum miR-193a-5p levels correlate strongly with NAFLD activity grade and fibrosis stage. MiR-193a-5p may have a role in the hepatic response to oxidative stress and is a potential clinically tractable circulating biomarker for progressive NAFLD.

2022-01-31 | GSE185062 | GEO

Adipocyte Precursor Cells from First Degree Relatives of type 2 diabetic patients feature changes of hsa-mir-23a-5p, -193a-5p, and -193b-5p and Insulin-Like Growth Factor 2 expression

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-11-19 | GSE162135 | GEO

U2OS osteosarcoma cells LC-MSMS

Project description:In order to identify the targets of miR-193a-5p in osteosarcoma U2OS cell line, we used a lentivirus-mediated expression system to overexpressing miR-193a precusor, miR-193a-5p target sequence and non-target sequence, respectively, in osteosarcoma cell line U2OS. A tandem mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-193a-5p-regulated proteins. order to identify the targets of miR-193a-5p, we used a lentivirus-mediated expression system to overexpressing miR-193a precusor, miR-193a-5p target sequence and non-target sequence, respectively, in osteosarcoma cell line U2OS. A tandem mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-193a-5p-regulated proteins.

2021-09-08 | PXD013987 | Pride

miR-193b-5p and miR-374b-5p are aberrantly expressed in endometriosis and suppress endometrial cell migration in vitro

Project description:miR-193b-5p and miR-374b-5p are aberrantly expressed in endometriosis and suppress endometrial cell migration in vitro

| PRJNA1174758 | ENA

miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro

Project description:miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro

| PRJNA1148930 | ENA

miR-193b-5p and miR-374b-5p Are Aberrantly Expressed in Endometriosis and Suppress Endometrial Cell Migration In Vitro

Project description:(1) Background: Endometriosis is a prevalent gynecological disease affecting 1 out of 10 women of reproductive age. MicroRNAs (miRNAs) may play a role in endometriosis, though their exact function is unclear. In this study we aimed to identify differentially expressed miRNAs in endometriosis and study their functional role in the disease. (2) Methods: Endometrial tissue was collected from patients with endometriosis (n=15) and healthy controls (n=17). Dysregulated miR-NAs were identified through small RNA-sequencing, and their function were explored by target gene prediction and pathway analysis. Selected miRNAs were validated in a cohort of paired ec-topic endometriomas and eutopic endometrium (n=10) using RT-qPCR. Their role in cell migration and proliferation were further examined in vitro using functional assays. To identify potential target genes, we performed mRNA sequencing of the miRNA-transfected cells and endometrioma cohort. (3) Results: We identified 14 dysregulated miRNAs in eutopic endometrium from women with en-dometriosis. Pathway analysis indicated enrichment in cell migration and proliferation pathways. miR-193b-5p and miR-374b-5p, selected for validation, were up-regulated in endometrioma. Over-expression of these two miRNAs in-vitro inhibited cell migration and mRNA sequencing further revealed that several genes related to cell migration are targeted by these miRNAs. (4) Conclusions: Our study identified two key endometrial miRNAs that may be involved in the pathogenesis of endometriosis by regulating cell migration.

2024-10-29 | GSE275002 | GEO

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