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Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM

ABSTRACT: Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM

PROVIDER: PRJNA681399 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13163311.fastq.gz	Fastqsanger.gz
	SRR13163312.fastq.gz	Fastqsanger.gz

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Explored the underlying mechanism by which ILF3 exerted carcinogenesis and its potential targets in MM

Project description:An RIP experiment was performed in ARP1 cell following the instructions of the article. ~5-20 × 10^6 cells were harvested and lysed. Protein A/G MagBeads pre-coated with 5μg of the antibody of interest (HNRNPA2B1/ILF3, Proteintech) and incubated with cell lysate supernatant overnight at 4°C.The beads containing immunoprecipitated RNA-protein complex were treated with 150μL of Proteinase K buffer to digest the proteins. Specific binding RNAs were isolated by using TRIzol.

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