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ABSTRACT: Fracture callus macrophages in old and young mice

PROVIDER: PRJNA682234 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13187700.fastq.gz	Fastqsanger.gz
	SRR13187701.fastq.gz	Fastqsanger.gz
	SRR13187702.fastq.gz	Fastqsanger.gz
	SRR13187703.fastq.gz	Fastqsanger.gz
	SRR13187704.fastq.gz	Fastqsanger.gz

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Project description:Immune cells are essential for proper fracture healing. The goal of this study was to compare the transcriptional profiles of the CD45+ immune cells isolated from the healing fracture callus of old (24 month) and young (3 month) mice (C57BL/6). Analysis of all CD45+ cells was completed via single cell RNAseq. Additional analysis of the macrophages involved in fracture healing in old and young mice was performed via bulk RNAseq. We show that there were multiple subpopulations of macrophages that were transcriptionally heterogenous and present simultaneously within the fracture callus tissue. Attenuated healing in the old mice was associated with a transcriptional profile that shifted towards more pro-inflammatory phenotypes.

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