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ABSTRACT: Effective double-digest RAD sequencing and genotyping despite large genome size

PROVIDER: PRJNA682386 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR13192418_1.fastq.gz	Fastqsanger.gz
	SRR13192418_2.fastq.gz	Fastqsanger.gz
	SRR13192419_1.fastq.gz	Fastqsanger.gz
	SRR13192419_2.fastq.gz	Fastqsanger.gz
	SRR13192420_1.fastq.gz	Fastqsanger.gz

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Project description:Restriction site Associated DNA (RAD) tags are a genome-wide representation of every site of a particular restriction enzyme by short DNA tags. Most organisms segregate large numbers of DNA sequence polymorphisms that disrupt restriction sites, which allow RAD tags to serve as genetic markers spread at a high-density throughout the genome. Here, we demonstrate the applicability of RAD markers for both individual and bulk-segregant genotyping. First, we show that these markers can be identified and typed on pre-existing microarray formats. Second, we present a method that uses RAD marker DNA to rapidly produce a low-cost microarray genotyping resource that can be used to efficiently identify and type thousands of RAD markers. We demonstrate the utility of the former approach by using a tiling path array for the fruit fly to map a recombination breakpoint, and the latter approach by creating and utilizing an enriched RAD marker array for the threespine stickleback. The high number of RAD markers enabled localization of a previously identified region, as well as a second novel region also associated with the lateral plate phenotype. Taken together, our results demonstrate that RAD markers, and the method to develop a RAD marker microarray resource, allow high-throughput, high-resolution genotyping in both model and non-model systems. Keywords: microarray genotyping

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