Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular diversity of olfactory bulb projection neurons [single-cell RNA-seq]

ABSTRACT: Molecular diversity of olfactory bulb projection neurons [single-cell RNA-seq]

PROVIDER: PRJNA682579 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13198220.fastq.gz	Fastqsanger.gz
	SRR13198221.fastq.gz	Fastqsanger.gz
	SRR13198222.fastq.gz	Fastqsanger.gz
	SRR13198223.fastq.gz	Fastqsanger.gz
	SRR13198224.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 21

Similar Datasets

Molecular diversity of olfactory bulb projection neurons

Project description:Molecular diversity of olfactory bulb projection neurons

| PRJNA682577 | ENA

Molecular diversity of olfactory bulb projection neurons [single-cell RNA-seq]

Project description:To characterize the molecular diversity of olfactory bulb projection neurons we used viral targeting and Fluorescence Activated Nuclei Sorting (FANS) to enrich for olfactory bulb projection neurons, and single-nuclei RNA sequencing (sn-RNA seq) to comprehensively characterize their transcriptomes. To isolate GFP-labelled nuclei, 3 individual replicates of AON and PCx-injected mice were used. Ipsilateral and controlateral sides were minced separately and placed into two different tubes. The minced tissue was gently homogenized in Nuclei PURE Lysis Buffer and 10% Triton X-100 using an ice-cold dounce and pestle, and filtered two times through a 40 μm cell strainer on ice. After centrifuging at 500 rpm for 5 min at 4 °C, the supernatant was aspirated and gently resuspended in 500 μl of cold buffer (1x of cold Hanks' Balanced Salt Solution HBSS, 1% nuclease-free BSA, RNasin Plus and 1/2000 DRAQ5). Our study identifies molecularly distinct subtypes of mitral and tufted cells.

2020-12-05 | GSE162654 | GEO

Retrograde labelling and RNA deep sequencing of olfactory bulb projection neurons [bulk RNA-seq]

2020-12-05 | GSE162655 | GEO

Validation of molecularly-defined olfactory bulb projection neurons targeting anterior or posterior brain areas

Project description:To validate different projection targets of already molecularly-defined olfactory bulb projection neurons we used viral targeting specifically into anterior or posterior cortical areas, Fluorescence Activated Nuclei Sorting (FANS) to enrich for olfactory bulb projection neurons, and single-nuclei RNA sequencing (sn-RNA seq) To isolate GFP-labelled nuclei, 1 individual replicate of AON or PCx-injected mice was used. Ipsilateral and controlateral sides were minced separately and placed into two different tubes. The minced tissue was gently homogenized in Nuclei PURE Lysis Buffer and 10% Triton X-100 using an ice-cold dounce and pestle, and filtered two times through a 40 μm cell strainer on ice. After centrifuging at 500 rpm for 5 min at 4 °C, the supernatant was aspirated and gently resuspended in 500 μl of cold buffer (1x of cold Hanks' Balanced Salt Solution HBSS, 1% nuclease-free BSA, RNasin Plus and 1/2000 DRAQ5). Our study identifies molecularly distinct subtypes of mitral cells projecting to anterior or posterior olfactory cortices.

2021-06-09 | GSE176350 | GEO

Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

Project description:Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

| PRJNA771410 | ENA

RNA sequencing of olfactory bulb projection neurons in neurotensin-GFP (NTS-GFP) mice at different developing time points

Project description:Characterize the spatiotemporal dynamics of gene expression in neurons in developing olfactory bulb

2016-04-15 | GSE70896 | GEO

Pathological consequences of chronic olfactory inflammation on neurite morphology of olfactory bulb projection neurons

Project description:The effects of chronic olfactory inflammation on the olfactory bulb (OB) were examined. Inflammation was induced in the olfactory epithelium by repeated unilateral intranasal administration of lipopolysaccharide (LPS). After 4 weeks, gene expression profiles were compared between ipislateral and contralateral OBs using RNA sequencing analysis.

2022-04-06 | GSE185945 | GEO

Retrograde labelling and RNA deep sequencing of olfactory bulb projection neurons [bulk RNA-seq]

Project description:Retrograde labelling and RNA deep sequencing of olfactory bulb projection neurons [bulk RNA-seq]

| PRJNA682580 | ENA

Validation of molecularly-defined olfactory bulb projection neurons targeting anterior or posterior brain areas

Project description:Validation of molecularly-defined olfactory bulb projection neurons targeting anterior or posterior brain areas

| PRJNA735939 | ENA

Mouse olfactory sensory neurons, main olfactory epithelium and main olfactory bulb

Project description:Mouse olfactory sensory neurons, main olfactory epithelium and main olfactory bulb

| PRJNA714906 | ENA