Ontology highlight
ABSTRACT:
PROVIDER: PRJNA682653 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR13201064_1.fastq.gz | Fastqsanger.gz | |||
| SRR13201064_2.fastq.gz | Fastqsanger.gz | |||
| SRR13201065_1.fastq.gz | Fastqsanger.gz | |||
| SRR13201065_2.fastq.gz | Fastqsanger.gz | |||
| SRR13201066_1.fastq.gz | Fastqsanger.gz |
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Project description:Small RNA libraries made from the rdr2/mop1 maize mutant, using RNA extracted from young ears (3 to 5 cm in length). Four libraries were constructed representing biological replicates. The mop1 mutant is depleted for heterochromatic siRNAs and enriched for 22-nt siRNAs of unknown function (as shown by Nobuta et al., 2008). These libraries were made in ~2010 but used for this study to assess the properties of the 22-nt, mop1-independent siRNAs.
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Project description:S. reilianum triggered loss of organ and meristem identity, and loss of meristem determinacy in male and female inflorescences and flowers. Microarray analysis showed that these developmental changes were accompanied with transcriptional regulation of genes proposed to regulate floral organ and meristem identity, and meristem determinacy in maize. Infected ears were compared with mock infected ears. Each treatment had 3 biological replicates Disease: S. reilianum-infected ears with elongated kernel: Zm_Sr5_15_2_Leafy ears4weeks_I, Zm_Sr5_15_2_Leafy ears4weeks_II, Zm_Sr5_15_2_Leafy ears4weeks_III Disease: Mock infected ears at: Zm_Sr5_15_2_Mock4weeks_I, Zm_Sr5_15_2_Mock4weeks_II, Zm_Sr5_15_2_Mock4weeks_III biological replicate: Zm_Sr5_15_2_Leafy ears4weeks_I, Zm_Sr5_15_2_Leafy ears4weeks_II, Zm_Sr5_15_2_Leafy ears4weeks_III biological replicate: Zm_Sr5_15_2_Mock4weeks_I, Zm_Sr5_15_2_Mock4weeks_II, Zm_Sr5_15_2_Mock4weeks_III
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Project description:This study looked for signals produced in UV-B irradiated leaves, and possibly induced in shielded leaves, that modulate physiological responses in maize. Transcriptome and proteomics profiling tracked changes in exposed and shielded organs. Metabolic profiling was examined for signaling molecules. Exposure of just the top leaf substantially alters the transcriptome of both irradiated and shielded organs, with greater changes as an additional 1-2 leaves are irradiated. Transcriptome, proteome and metabolome changes are UV-B regulated in shielded organs. Early steps in signal transduction and possible signal molecules are identified utilizing a time course experiment. Keywords: UVB, maize, leaves, ears
2011-02-01 | GSE25038 | GEO
Project description:This study looked for signals produced in UV-B irradiated leaves, and possibly induced in shielded leaves, that modulate physiological responses in maize. Transcriptome and proteomics profiling tracked changes in exposed and shielded organs. Metabolic profiling was examined for signaling molecules. Exposure of just the top leaf substantially alters the transcriptome of both irradiated and shielded organs, with greater changes as an additional 1-2 leaves are irradiated. Transcriptome, proteome and metabolome changes are UV-B regulated in shielded organs. Early steps in signal transduction and possible signal molecules are identified utilizing a time course experiment. Keywords: UVB, maize, leaves, ears Compared irradiated leaves in a time course, irradiated leaves versus shielded leaves, and shielded ears on plants with irradiated leaves. Also compared different number of leaves being irradiated. Spike-in controls were included.
2011-02-01 | E-GEOD-25038 | biostudies-arrayexpress
Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different maize tissues (including leaves, ears and tassels) collected from wild-type plants of the B73 variety. The high depth of the resulting datasets enabled us to examine in detail critical small RNA features as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the maize genome under study. Small RNA libraries were derived from leaves, ears and tassels of maize variety B73 (wild-type). Plants were grown in a flood irrigated plot at the University of Arizona (Tucson, AZ, USA) in 2007 and organs were pooled from several plants for each library. Young leaves were collected from 6-weeks-old seedlings. Post-meiotic immature ears were harvested from 10- and 11-week old plants while pre-meiotic tassels were collected from 8-week old plants. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen) and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Lyudmila Sidorenko and Vicki Chandler for providing the plant material and Kan Nobuta for assistance with the computational methods.
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