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Berghia stephanieae

ABSTRACT: Draft Genome of the photosymbiotic nudibranch Berghia stephanieae Raw sequence reads

PROVIDER: PRJNA684024 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13242883_1.fastq.gz	Fastqsanger.gz
	SRR13242884.fastq.gz	Fastqsanger.gz
	SRR13242885.fastq.gz	Fastqsanger.gz

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Berghia stephanieae

Project description:Berghia stephanieae overview

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High-throughput single-cell transcriptomes of the brain of the nudibranch Berghia stephanieae

Project description:Previously, transcriptomics data for mollusc has been obtained by whole-brain bulk RNA-seq and low-throughput scRNA-seq. We want to construct the first molluscan high-throughput single-neuron transcriptomes for Berghia stephanieae. Around 129,000 cells were collected from 20 brains and the libraries were constructed using the 10X Genomics' Chromium platform. The brains were separated into two samples: the main brain (i.e., the cerebropleural, pedal, buccal ganglion) and the rhinophore ganglion sample. After library preparation, around 1,000 cells were receovered and sequenced. After data analysis, the cells formed eight clusters with marker genes for each cluster identified. Various cell populations that express a wide range of both small-molecule neurotransmitters and neuropeptides such as serotonergic, small cardioactive peptide (SCP), APGWamide, and FMRFamide cells were also identified in the dataset. Interestingly, cells from the rhinophore ganglion of Berghia exhibit great cell heterogeneity, with cells splitting into two general categories and four distinct clusters. The project produced a single-cell dissociation protocol that can be adapted for use in other nudibranch molluscs and a custom data analysis pipeline for data of this nature.

2022-01-14 | GSE165967 | GEO

Berghia stephanieae

Project description:Microbiome of Exaiptasia diaphana and Berghia stephanieae fed and under different starvation conditions

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High-throughput single-cell transcriptomes of the brain of the nudibranch Berghia stephanieae

Project description:High-throughput single-cell transcriptomes of the brain of the nudibranch Berghia stephanieae

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Berghia rissodominguezi

Project description:Berghia rissodominguezi

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Project description:Transcriptome of the aeolid nudibranch Berghia stephanieae in symbiotic and aposymbiotic states

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State-dependent, visually guided behaviors in the nudibranch Berghia stephanieae.

Project description: Not available

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Prevalence and Photobiology of Photosynthetic Dinoflagellate Endosymbionts in the Nudibranch Berghia stephanieae.

Project description: Not available

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A draft genome sequence of the elusive giant squid, Architeuthis dux

Project description:We present a draft genome assembly that includes 200 Gb of Illumina reads, 4 Gb of Moleculo synthetic long-reads and 108 Gb of Chicago libraries, with a final size matching the estimated genome size of 2.7 Gb, and a scaffold N50 of 4.8 Mb. We also present an alternative assembly including 27 Gb raw reads generated using the Pacific Biosciences platform. In addition, we sequenced the proteome of the same individual and RNA from three different tissue types from three other species of squid species (Onychoteuthis banksii, Dosidicus gigas, and Sthenoteuthis oualaniensis) to assist genome annotation. We annotated 33,406 protein coding genes supported by evidence and the genome completeness estimated by BUSCO reached 92%. Repetitive regions cover 49.17% of the genome.

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