Project description:The transcriptomics changes induced in the human liver cell line HepG2 by 17 hepatotoxic compounds, 5 non-hepatotoxic compounds and solvent controls after treatment for 24h The study investigated differential gene expression in HepG2 cell line mRNA following 24 hours of exposure to 17 hepatotoxic compounds, 5 non-hepatotoxic compounds and solvent controls. Three biological replicates per compound/solvent. In total 105 arrays .
Project description:The transcriptomics changes induced in the human liver cell line HepG2 by 17 hepatotoxic compounds, 5 non-hepatotoxic compounds and solvent controls after treatment for 24h
Project description:HepG2 cells were treated with 50 uM fangchinoline for 24 h. Proteomic profile of fangchinoline-treated HepG2 cells was compared with untreated cells.
Project description:The transcriptomics-based in vitro assay for predicting chemical genotoxicity in vivo published by Magkoufopoulou et al (2012) was validated by the independent study performed in HepG2 cells for which the gene expression data are described here. The data were reanalyzed for the OpenRiskNet project (Horizon2020 EINFRA-22-2016 Programme; Grant Agreement 731075).
Project description:The lack of accurate in vitro assays for predicting in vivo toxicity of chemicals together with new legislations demanding replacement and reduction of animal testing has triggered the development of alternative methods. This study aimed at developing a transcriptomics-based in vitro prediction assay for in vivo genotoxicity. The transcriptomics changes induced in the human liver cell line HepG2 by 34 compounds after treatment for 12h, 24h and 48h were used for the selection of gene-sets that can discriminate between in vivo genotoxins (GTX) and in vivo non-genotoxins (NGTX). By combining publicly available results for these chemicals from standard in vitro genotoxicity studies with transcriptomics, we developed several prediction models. These models were validated by means of an additional set of 28 chemicals. The study investigated differential gene expression in HepG2 cell line mRNA following 12 hours of exposure to 34 different compounds and their solvents; 24 and 48 hours of exposure to 62 different compounds and their solvents. Three biological replicates per compound/solvent. In total 560 arrays .
Project description:HepG2 cells were exposed for 24 hours to the IC20 (72hr MTT) dose of various pesticides and related compounds selected from the EPA Toxcast phase 1 set. This is the first part of two batches of experiments, these experiments were used as a training set and the second batch as a validation set.
Project description:This study provides an evaluation of changes in gene expression associated with treating human HEPG2 cells with 34 different chemical compounds. Gene expression experiments studies were performed in triplicate (n=3) with the cells for each replicate treated and harvested on separate days. This series is part of a SuperSeries in which human toxicology-relevant cell lines were treated with three doses of 34 chemical compounds (and corresponding vehicle controls) for 6 hours. Each compound/vehicle treatment group was an individual study performed at different times. Each study was analyzed separately and themes common between studies were reported.
Project description:This study provides an evaluation of changes in gene expression associated with treating human HEPG2 cells with 34 different chemical compounds. Gene expression experiments studies were performed in triplicate (n=3) with the cells for each replicate treated and harvested on separate days.