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Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [bulk RNA-seq]

ABSTRACT: Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [bulk RNA-seq]

PROVIDER: PRJNA687688 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13302400.fastq.gz	Fastqsanger.gz
	SRR13302401.fastq.gz	Fastqsanger.gz
	SRR13302402.fastq.gz	Fastqsanger.gz
	SRR13302403.fastq.gz	Fastqsanger.gz
	SRR13302404.fastq.gz	Fastqsanger.gz

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Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [bulk RNA-seq]

Project description:Purpose: The goal of this study is to determine alterations in heart endothelial cell transcripts associated with aging. This is accomplished by comparing endothelial transcriptomes from young and aged organs (heart, brain and kidney). Methods: Endothelial mRNA profiles of 3 month and 24 month old wild-type mice were generated by next generation sequencing using libraries generated via SmartSeq V4 chemistry (Takara) and sequenced on an HiSeq 2500 (Illuina). Tophat2 with default parameters was used to align the sequenced reads against the mouse genome using the GRCm38. HTSeq python software with default parameters was used to quantify the transcripts of the aligned reads using the corresponding GRCm38 gene annotation model from Ensembl. Results: Highly enriched populations of endothelial cells (ECs) isolated from the heart, brain and kidney of young (3 months) and old (24 months) C57/BL6 mice were profiled for RNA expression via bulk RNA sequencing. Approximately 700 cardiac endothelial transcripts significantly differ by age. Gene set enrichment analysis indicated similar patterns for cellular pathway perturbations. Receptor-ligand comparisons indicated parallel alterations in age-affected circulating factors and cardiac endothelial-expressed receptors. Single-cell RNA-seq analysis identified 9 distinct endothelial cell subtypes in the heart with an age-associated population shift observed for an Aplnr-enriched endothelial cell clusters. Conclusions: Gene and pathway enrichment analyses show that age-related transcriptional response of cardiac endothelial cells is distinct from that of endothelial cells derived from the brain or kidney vascular bed. Furthermore, single-cell analysis identified 9 distinct EC subtypes, and shows that Aplnr-enriched subtype is reduced with age in mouse heart. Finally, we identify age-dysregulated genes in specific aged cardiac endothelial subtypes.

2021-05-28 | GSE163821 | GEO

Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells

Project description:Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells

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Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [scRNA-seq]

Project description:Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [scRNA-seq]

| PRJNA687689 | ENA

Age-Dependent Transcriptional Alterations in Cardiac Endothelial Cells [scRNA-seq]

Project description:Purpose: The goal of this study is to determine alterations in heart endothelial cell transcripts and cell populations associated with aging. Methods: Endothelial single cell mRNA profiles of 3 month and 24 month old wild-type mice were generated by 10X Genomics Results: Using an optimized data analysis workflow, we mapped about 50 thousand reads per cell Conclusions: Our study represents the first analysis of aged cardiac endothelial cell transcriptomes using single cell RNA-seq technology, identifiying specific transcripts and cell populations affected by age.

2021-05-28 | GSE163822 | GEO

Untreated Rat Cardiac Ventricular tissue Age

Project description:The objective of this study was to investigate changes in rat cardiac ventricle transcriptome with age. It was run along similar with other tissues to look for common ageing signatures. Particular interest would be cardiomyocytes, but this is bulk RNA-sequencing.

2025-09-02 | GSE272911 | GEO

Project description:Age-related alterations in meningeal immunity drive impaired CNS lymphatic drainage [bulk LECs]

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RNA-sequencing of endothelial cells and 3D cardiac microtissues from hiPSCs

Project description:We previously showed that two major cellular components of the human heart, cardiac endothelial cells and cardiomyocytes, could be derived simultaneously from human induced pluripotent stem cells (hiPSCs). In vivo studies in mice have shown these lineages derive from a common MESP1+ mesoderm progenitor. Although this has been investigated using whole transcriptome sequencing, the transcriptional control and dynamics of their segregation from cardiac mesoderm are not yet fully understood. In addition, the same transgenic approach cannot be used in human. Here, we used bulk and single cell RNA sequencing (RNAseq) to investigate EC and cardiomyocytes co-differentiation in hiPSC. We confirmed segregation of these two cardiac lineages from common cardiac mesoderm precursors but more importantly, revealed a critical role of transient expression of the transcription factor ETV2 for endothelial fate specification and showed unexpectedly that functional cardiomyocytes could also originate from ETV2+ progenitors. This dataset contain 10X-based scRNA-seq data human induced pluripotent stem cell derived endothelial cells and 3D cardac microtissues, as well as bulk RNA-seq data of endothelial cells derived from cardiac and paraxial mesoderm.

2025-08-20 | GSE151427 | GEO

Untreated Rat Cardiac Ventricular tissue Age

Project description:Untreated Rat Cardiac Ventricular tissue Age

| PRJNA1139213 | ENA

Bulk RNA-sequencing of VE-Cadherin+/CD45- mouse aorta endothelial cells

Project description:In mouse aorta endothelial cells, populations of endothelial vascular progenitor (EVP) and differentiated (D) cells could be identified by CD31 (lo/hi) and VEGFR2 (lo/hi) expression. These populations were FACS sorted and paired-end bulk RNA-sequencing was performed.

2019-05-27 | E-MTAB-7148 | biostudies-arrayexpress

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2025-10-31 | GSE298225 | GEO

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