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ThPOK is a critical and multifaceted regulator of myeloid lineage development [CITE-Seq]

ABSTRACT: ThPOK is a critical and multifaceted regulator of myeloid lineage development [CITE-Seq]

PROVIDER: PRJNA687741 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR13302748.fastq.gz	Fastqsanger.gz
	SRR13302749.fastq.gz	Fastqsanger.gz
	SRR13302750.fastq.gz	Fastqsanger.gz
	SRR13302751.fastq.gz	Fastqsanger.gz

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ThPOK is a critical and multifaceted regulator of myeloid lineage development [CITE-Seq]

Project description:The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report a novel role for ThPOK as a critical and multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with CITE-Seq, Smart-Seq2, progenitor transfer and clonogenic assays and we show that ThPOK controls monocyte-DC versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered mRNA splicing to reprogram intron retention.

2023-06-05 | GSE163837 | GEO

ThPOK is a critical and multifaceted regulator of myeloid lineage development [Fluidigm]

2023-06-05 | GSE163843 | GEO

ThPOK is a critical and multifaceted regulator of myeloid lineage development

Project description:ThPOK is a critical and multifaceted regulator of myeloid lineage development

| PRJNA687746 | ENA

ThPOK is a critical and multifaceted regulator of myeloid lineage development [Fluidigm]

Project description:ThPOK is a critical and multifaceted regulator of myeloid lineage development [Fluidigm]

| PRJNA687742 | ENA

ThPOK is a critical multifaceted regulator of myeloid lineage development.

Project description:The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout mouse models combined with single-cell RNA-sequencing, progenitor transfer and colony assays, we show that ThPOK controls monocyte-dendritic cell versus granulocyte lineage production during homeostatic differentiation, and serves as a brake for neutrophil maturation in granulocyte lineage-specified cells through transcriptional regulation of lineage-specific transcription factors and RNA via altered messenger RNA splicing to reprogram intron retention.

| S-EPMC10792516 | biostudies-literature

ThPOK is a critical and multifaceted regulator of myeloid lineage development

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-06-05 | GSE163844 | GEO

Gene Expression in Effector T cells, wildtype of Thpok-deficient

Project description:The transcription factor Thpok is essential for CD4 T cell development in the thymus and remains expressed in post-thymic CD4 T cells. We post-thymically inactivated Thpok and compared microarray gene expression in Thpok-deficient CD4 T cells to that in their wildtype CD4 or CD8 counterparts We show that Thpok constrains the transcriptional circuitry to maintain CD4+-lineage integrity in naM-CM-/ve cells and to couple effector differentiation to environmental cues after antigenic stimulation. Redundantly with the related factor LRF, Thpok is continuously needed to prevent the trans-differentiation of mature CD4+ into -CD8+ T cells. We activated naM-CM-/ve CD4 T cells (either wild-type or Thpok-deficient) and CD8 T cells (wild-type) in vitro under Th1 conditions. Differentiated effectors were sorted 4 days after activation into CD4+CD8- and CD4-CD8+ (wild-type) and CD4+CD8- and CD4+CD8+ (Thpok-deficient) subsets. Total RNA was extracted from sorted subsets and processed for microarray analyses (Affymetrix Mouse Exon 1.0 ST array) at the NCI microarray facility, following the manufacturerM-bM-^@M-^Ys recommendation. Data is from 3 replicates (except wild-type CD4-CD8+ cells, for which two samples only were processed), generated from two distinct cell preparations.

2014-08-15 | E-GEOD-57846 | biostudies-arrayexpress

Kaiso, a critical regulator of spermatogenesis

Project description:Kaiso, a critical regulator of spermatogenesis

| PRJNA436947 | ENA

Super-enhancer profiling reveals ThPOK/ZBTB7B, a CD4+ cell lineage commitment factor, as a breast cancer master regulator that restricts cells to a luminal non-migratory phenotype

Project description:Despite considerable efforts to understand the biology of breast cancer, metastatic disease remains a clinical challenge. Identifying suppressors of breast cancer progression and mechanisms of transition to more invasive phenotypes could provide game changing therapeutic opportunities. Transcriptional deregulation is central to all malignancies, highlighted by the extensive reprogramming of regulatory elements that underlay oncogenic programs. Among these, super-enhancers (SEs) stand out due to their enrichment in genes controlling cancer hallmarks. To reveal novel breast cancer dependencies, we integrated the analysis of the SE landscape with master regulator activity inference for a series of breast cancer cell lines. As a result, we identified T-helper-inducing Poxviruses and Zinc-finger (POZ)/Krüppel-like factor (ThPOK, ZBTB7B), a CD4+ cell lineage commitment factor, as a breast cancer master regulator that is recurrently associated with a SE. ThPOK expression is highest in luminal breast cancer, but is significantly reduced in the basal subtype. Manipulation of ThPOK levels in cell lines shows that its repressive function restricts breast cancer cells to the luminal subtype by suppressing the expression of genes involved in the epithelial-mesenchymal transition (EMT), WNT/b-catenin pathway, and the pro-metastatic TGFb. Our study reveals ThPOK as a breast cancer master transcription factor with opposing forces to the acquisition of metastatic features.

2025-07-01 | GSE207245 | GEO

Essential role of ThPOK autoregulatory loop in maintenance of mature CD4 T cell identity and function [RNA-seq]

Project description:The transcription factor ThPOK is critical for homeostasis and differentiation of mature T helper cells. Overall goal of this experiment is to show divergent gene expression profiles of ThPOK-dependent autoregulatory loop disrupted in CD4 T cells.

2021-04-05 | GSE168770 | GEO

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