Project description:<p>Entomopathogenic fungi have the ability to both directly kill insect pests and act as plant endophytic fungi to impact plant growth and development. Despite this, the widespread endophytic use of these fungi in tea plants (Camellia sinensis) is still limited. This study examined how Beauveria bassiana colonizes tea plant tissues and its impact on tea plant growth and development. Through amplicon sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS), the study investigated the response patterns of dominant endophytic microbial populations in tea plants during fungal colonization and the alterations in secondary metabolites in tea plants due to the colonization process. B. bassiana effectively colonized tea seedlings through root irrigation and foliar spraying methods, showing a preference for stems, and the colonization persisted for more than 90 days. Colonization resulted in a reduction in the diversity and structural stability of the endophytic microbial community in tea plants; however, it also enhanced the importance of ecologically mutualistic relationships or cooperative interactions in community assembly. Additionally, colonization had a more pronounced effect on endophytic fungi compared to endophytic bacteria. It led to an increase in the relative abundance of arbuscular mycorrhizal fungi in root tissues and a decrease in the relative abundance of total plant pathogens in tea plant tissues. Terpenoids were the most significantly associated differential metabolites following B. bassiana colonization, followed by steroids and their derivatives, and flavonoids. Colonization by B. bassiana resulted in elevated levels of the majority of differential metabolites in tea plant stems at the onset of colonization. The colonization of B. bassiana in tea plants displayed a strong positive correlation with 26 distinct metabolites, such as proanthocyanidin B2 and L-malic acid. This colonization altered the endophytic microbial community, impacting metabolic pathways associated with plant hormone synthesis, volatile compound production, as well as the growth, development, and defense of tea plants.</p>
Project description:Next generation sequencing (NGS) was performed to identify genes changed in tea plant upon Colletotrichum camelliae infection. The goal of the work is to find interesting genes involved in tea plant in response to fungi infection. The object is to reveal the molecular mechanism of tea plant defense.
Project description:Next-generation sequencing (NGS) was performed to identify genes changed in tea plant cultivar Zhongcha 108 upon Colletotrichum camelliae infection. The goal of the work is to find interesting genes involved in tea plant in response to fungi infection. The object is to reveal the molecular mechanism of tea plant defense.
Project description:In this study, it is noticeable that 32 tea-specific miRNAs were confirmed on the base of genome survey, using deep sequencing and microarray hybridization, and many miRNAs might associate with secondary metabolites synthesis. Leaves, buds and roots were collected
Project description:Senescence is initiated immediately in harvested tea leaves, and leads to physiological and biochemical changes, and could affects the final tea products. In the present work, we investigated the relationship between hormones and critical components in harvested tea leaves before withering, changes in hormones including abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and critical components like catechins, theanine, and caffeine were analyzed. Significant changes in these substances were identified and ABA correlated with catechin in harvested tea leaves before withering. RNA-seq transcriptome analysis revealed dramatic differences between tea samples at 1 h and 2 h compared with those at 0 h. The patterns of these three critical components correlated with the expression profiles of differentially expressed genes (DEGs). Weighted correlation network analysis of co-expressed genes revealed that genes in the mediumpurple2 module correlated with ABA and catechins. The results of this study suggest that harvested tea leaves before withering undergo significant hormonal changes (ABA, JA, and SA) and ABA may participate in regulating catechin biosynthesis.
Project description:In this study, we performed deep sequencing and bioinformatics analyses of tea plant leaves to identify and characterize known and novel miRNAs. A total of 26,876,261 raw reads were produced from 2 libraries. We detected 422 known miRNAs belonging to 125 families, and 68 putative novel miRNAs.
Project description:Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the cold treatment of tea leaves. Subsequently, aligning these sequencing date with plant known miRNAs, we characterized 112 C. sinensis conserved miRNAs. In addition, 215 potential candidate miRNAs were found; among them, 131 candidates with star sequence were chosen as novel miRNAs. There are both congruously and differently regulated miRNAs, and line-specific miRNAs were identified by microarray-based hybridization in response to cold stress. The miRNA chip included 3228 miRNA probes corresponding to miRNA transcripts listed in Sanger miRBase release 19.0 and 283 novel miRNAs probes founding in tea plant. In the study presented here, two tea plant cultivars, ‘Yingshuang’ (YS, a cold-tolerant tea plant cultivar) and ‘Baiye 1’ (BY, a cold-sensitive tea plant cultivar), were kept at 4°C for 4,12, 24 h, respectively, and 28°C for as control. These samples were used to acquire expression profiles of a total of 3,511 unique genes, leading to the successful construction of supervised
