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OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency

ABSTRACT: OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency

PROVIDER: PRJNA688322 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13293413.fastq.gz	Fastqsanger.gz
	SRR13293414.fastq.gz	Fastqsanger.gz
	SRR13293415.fastq.gz	Fastqsanger.gz
	SRR13293416.fastq.gz	Fastqsanger.gz
	SRR13293417.fastq.gz	Fastqsanger.gz

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OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [Group1-4]

Project description:OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [Group1-4]

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OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [ATAC-Seq]

Project description:OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [ATAC-Seq]

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OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [RNA-Seq]

Project description:OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [RNA-Seq]

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OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [CUT&RUN]

Project description:OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [CUT&RUN]

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OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [CUT&RUN]

Project description:The transcription factors OCT4 and SOX2 play an essential role in the establishment and maintenance of pluripotent embryonic stem cells (ESCs). Yet, their function in specialized stem cell populations is still poorly understood. Here, we show that the OCT4 and SOX2 work as dimers to regulate the epigenomic landscape of neural crest cells. By isolating primary neural crest cells at a range of developmental stages, we characterized the transcriptomic and epigenomic changes that take place during specification, migration, and early differentiation. Analysis of these datasets revealed that the OCT4/SOX2 dimer promotes an epigenomic signature inherent to the multipotent neural crest. We found that the emergence of this epigenomic state requires the translocation of OCT4/SOX2 to tissue-specific cis-regulatory regions. By examining genome organization during the induction of hESCs into neural crest cells, we observed that the patterns of genomic occupancy of the dimer are modified during cell fate commitment. Dimer translocation is guided by neural crest-specific pioneer transcription factors, which physically interact with the OCT4/SOX to modify their genomic targets. Our results demonstrate how the ESC pluripotency program is repurposed in specialized stem cells to control chromatin organization and define the developmental potential of embryonic progenitors.

2022-09-06 | GSE163960 | GEO

OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [ATAC-Seq]

2022-09-06 | GSE163771 | GEO

OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [RNA-Seq]

2022-09-06 | GSE163768 | GEO

OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency [Group1-4]

2022-09-06 | GSE200196 | GEO

OCT4-SOX2 dimers reshape the epigenome to promote neural crest multipotency

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-09-02 | GSE163961 | GEO

Dissecting pluripotency through the different OCT4 and SOX2 assembly configurations

Project description:The transcription factors OCT4 and SOX2 are required for generating induced pluripotent stem cells (iPSCs) and for maintaining embryonic stem cells (ESCs). To this end, OCT4 and SOX2 associate and bind to DNA in different configurations depending on the composite DNA element present in their target genes. Here, we have investigated the role of the different OCT4-SOX2 conformational arrangements in regulating and inducing pluripotency. To this end, we have generated SOX2 mutants that interfere with specific OCT4-SOX2 heterodimer configurations and we have assessed their abilities to generate iPSCs and to rescue ESC self-renewal. Our results demonstrate that the OCT4-SOX2 configuration that dimerizes on a Hoxb1-like composite, a canonical element with juxtaposed individual binding sites, plays a more critical role in the induction and maintenance of pluripotency than other OCT4-SOX2 configurations. Overall, the results of this study provide a new insight into the protein interactions required to establish a de novo pluripotent network and to maintain a true pluripotent cell fate.

2017-02-13 | GSE65345 | GEO

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