Project description:SELEX (systematic evolution of ligands by exponential enrichment) and RNA Immunoprecipitation (RIP)-seq revealed that CIRBP directly binds the 3’UTR of Atp5g3.
Project description:SELEX (systematic evolution of ligands by exponential enrichment), chromatin immunoprecipitation (ChIP)-seq, and crosslinking immunoprecipitation (CLIP)-seq showed that the DDX43 KH domain binds C/T rich DNA and U rich RNA.
Project description:To identify the directly bound transcripts of Flag antibody (the backgroud control for our METTL16 RIP-seq), RNA immunoprecipitation sequencing (RIP-seq) was conducted HEK293T. Briefly, HEK293T cells were infected with pmiRNA1-empty vector. Only the GFP-positive cells were used for study and expanded in DMEM medium.
Project description:We applied high-throughput SELEX (HT-SELEX) to profile the DNA-binding specificity of 106 putative nucleic acid–binding proteins (NBPs) from 103 DNA viruses. Three NBPs derived from viruses whose hosts are not humans (YP_010782993.1, NP_671816.1, and AVM80381.1) were included as negative controls. We identified sequence motifs for 33 proteins.