Project description:We report the application of scRNA sequencing for high-throughput profiling of gene epxression in 40 unsupervised lung cell clusters
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling (RNA-seq) of intestinal progenitor cells in Drosophila wild type and Sc overexpressed midgut. Methods: mRNA profiles of intestinal progenitor cells isolated from 2-day-old wild-type (Ctrl) and Sc overexpressed(ScOE) Drosophila midgut were generated by deep sequencing using Illumina HiSeq 4000. Results:
Project description:This study investigated mechanisms of acquired resistance to FGFR inhibitor therapy in RAS/RAF–wild-type colorectal cancer (CRC). RNA sequencing (RNA-seq) data from 47 RAS/RAF-wild-type colorectal cancer stem-cell (CRC-SC) spheroid lines previously deposited under GSE205787 were integrated with newly generated RNA-seq data from 31 additional RAS/RAF-wild-type CRC-SC spheroid lines and seven FGFR inhibitor-resistant CRC-SC derivatives. The resistant derivatives were established from parental CRC-SC spheroid lines by continuous exposure to erdafitinib or futibatinib. Comparative transcriptomic analysis between matched parental and resistant lines revealed consistent upregulation of EGFR and downregulation of PTPROt, a truncated isoform of PTPRO, in resistant derivatives. Gene set enrichment analysis further indicated activation of EGFR-related signaling pathways in FGFR inhibitor-resistant spheroids. In addition, the integrated RNA-seq dataset comprising 78 RAS/RAF-wild-type CRC-SC lines was used to validate the inverse correlation between EGFR and PTPRO mRNA expression, supporting the involvement of PTPROt downregulation and EGFR pathway activation in acquired resistance to FGFR inhibition.
Project description:Mice are on a C57Bl/6J background strain, hearts were collected from Clock and WT mice at ZT07, following 24 weeks on either a HF or SC diet. The microarray approach allows the investigation of gene expression changes of all genes in Clock HFD vs. Clock SC vs. WT HFD vs. WT SC hearts.
Project description:Reference experiment of wild type Saccharomyces cerevisiae cells of the BJ5457 background. Cells were grown in synthetic complete medium (SC) with the addition of the necessary aminoacids and factors (leucine, uracil, histidine, tryptophane) and harvested in exponential phase (OD=0.7-1). Cells were grown in the presence of BCS and BPS chelators. <br>