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Temporal changes of gene expression in bovine endometrium after parturition

ABSTRACT: Temporal changes of gene expression in bovine endometrium after parturition

PROVIDER: PRJNA692453 | ENA |

REPOSITORIES: ENA

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Temporal changes of gene expression in bovine endometrium after parturition

Project description:To clarify the regenerative mechanism of endometrium after parturition in cows, mRNA expression profiles in bovine endometrium were investigated during postpartum period. after PVP-I treatment in cows. The differentially expressed genes in the endometrium between postpartum days 49-52 and days 99-101 were 23 genes, and they were much lower than those before postpartum days 49-52. This result suggests that endometrial regeneration after parturition is completely accomplished until postpartum days 49-52.

2021-01-16 | GSE164924 | GEO

Gene expression in bovine endometrium at different period after parturition

Project description:In order to clarify whether the term after calving is a factor to affect the gene expression in the endometrium of cows, we conducted global endometrial gene expression analysis at different period after parturition. In bovine endometrium between 60.1 ± 4.0 days and 388.9 ± 8.2 days after parturition, the gene expression pattern was similar and the differentially expressed (>2-fold difference, P<0.05) genes were only 4 genes.

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Changes in proportion of side population cells and gene expression in bovine endometrium after Intrauterine administration of povidone-iodine

Project description:To clarify whether the regenerative mechanism of endometrium after PVP-I treatment is similar to that after parturition in cows, mRNA expression profiles in bovine endometrium were investigated after PVP-I treatment in cows. Common differentially expressed genes between after PVP-I administration and parturition were few. The difference of the pattern of gene expression changes between after PVP-I administration and postpartum period suggests that the endometrial regeneration after PVP-I administration had different mechanism from parturition.

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Gene expression in bovine endometrium at different period after parturition

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Temporal changes in bovine milk microbiota

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Uterine infection-induced transcriptomic changes in the bovine endometrium

Project description:Purpose: Perform RNA-seq study on infectious bovine endometrial tissues to reveal important genes and biological pathways regulating uterine physiology following uterine infections Methods:RNA sequencings were done using Illumina platform. Single-end reads in the FASTQ format were explored using FastQC, low-quality reads were trimmed from both 3’ and 5’ ends until a base pair of Phred quality score of 30 (99.9% accurate) or greater was found, reads having a mean quality score less than 30 and length below 30 nucleotides were filtered out. Cleaned reads were aligned against the bovine reference genome (Bos_taurus.ARS-UCD1.2) using HiSAT2. The resulting SAM files were sorted, converted to BAM files using SAMtools. Read counts mapped to bovine gene models were generated using htseq-count script from HTSeq package. Bioconductor DESeq2 was used to get the differentially expressed genes among infectious vs normal uterine tract groups Conclusions: The study demonstrated that uterine infections altered several genes and pathways related to inflammatory response, immune response, uterine physiology, uterine enviroment and fertility in the intercaruncular region of bovine endometrium.

2022-07-28 | GSE209700 | GEO

Small RNA-seq of the bovine endometrium

Project description:For the establishment of a normal pregnancy, not only the pregnancy period but also the reproductive physiology of the estrous cycle is important. This is because the endometrium prepares for subsequent fertilization and implantation during the estrous cycle. However, the miRNA profile in the bovine endometrium with regard to stage-specific expression during the estrous cycle remains unexplored. Here, we performed small RNA sequencing analysis using bovine endometrial tissue from different stages of estrous cycle and pregnancy. As a result, differentially expressed miRNAs (DEMs) were identified between each stage, and the reproductive physiological functions in which those DEMs are involved were predicted.

2026-09-02 | GSE344204 | GEO

Effect of intrauterine MGO administration on bovine endometrium

Project description:To clarify the effect of intrauterine methylglyoxal (MGO) administration on the endometrium, mRNA expression profiles of bovine endometrium were investigated. Hierarchical cluster analysis with the expression levels of all genes was divided these cows into two clusters. First cluster was composed of control cows, second cluster contained MGO (5 mM) treated cows.

2021-01-19 | GSE165039 | GEO

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