Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA sequencing of healthy and inflamed murine neutrophils

ABSTRACT: Single-cell RNA sequencing of healthy and inflamed murine neutrophils

PROVIDER: PRJNA693858 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR13499409.fastq.gz	Fastqsanger.gz
	SRR13499410.fastq.gz	Fastqsanger.gz
	SRR13499411.fastq.gz	Fastqsanger.gz
	SRR13499412.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 5

Similar Datasets

Single-cell RNA sequencing of healthy and inflamed murine neutrophils

Project description:Dataset containing single-cell RNA sequencing data of >17,000 healthy and perturbed murine neutrophils across biological tissues. This study contains expression data from healthy tissues (bone marrow, spleen, peripheral blood) as well as models of experimental (K/BxN serum transfer arthritis: peripheral blood and joint, IL-1β peritonitis and IL-1β pneumonitis). Single cell RNA-sequencing was performed on the 10X platform

2021-02-10 | GSE165276 | GEO

Proteome of neutrophils upon migration towards inflamed synovial fluid

Project description:Analysis of neutrophil proteomic alterations induced by migration towards inflamed joints in juvenile idiopathic arthritis (JIA). In this experiment neutrophil proteomes were investigated after migration towards JIA synovial fluid in an in vitro model of a synovial membrane, compared to neutrophils incubated in synovial fluid without migration. The migration model consisted of transwell inserts with human knee synoviocytes on the undersides and HMEC endothelial cells on the insides, placed in wells containing medium with 10 % JIA synovial fluid.

2022-02-15 | PXD029129 | Pride

Bone morphogenic protein-4 availability in the cardiac microenvironment controls myocardial inflammation and fibrosis - Single cell transcriptome analysis from healthy and inflamed murine hearts

Project description:Myocarditis is a heart condition that causes inflammation and results in the loss of heart muscle cells, often leading to fibrosis (scarring) of the heart tissue and heart failure. However, the molecular mechanisms underlying immune cell control and maintenance of tissue integrity in the inflamed cardiac microenvironment remain elusive. Based on our finding that bone morphogenic protein-4 (BMP4) serum concentration was reduced in myocarditis patients in combination with comprehensive single cell and single nucleus RNA sequencing analyses of inflamed murine and human myocardial tissue indicated that BMP4 gradients maintain cardiac tissue homeostasis. Indeed, restoration of BMP signaling through antibody-mediated neutralization of the BMP-inhibitors GREM1 and GREM2 reduced CD4+ T cell-mediated myocardial inflammation and blocked disease progression by reduction of adverse fibrotic remodelling. These results unveil a key function of the BMP4-GREM1/2 axis as promising approach for treating myocardial inflammation and the serious complications of cardiac fibrosis and heart failure.

2024-01-16 | E-MTAB-12589 | biostudies-arrayexpress

Bone morphogenic protein-4 availability in the cardiac microenvironment controls myocardial inflammation and fibrosis - Single nucleus RNA sequencing data from healthy and inflamed murine hearts

2024-01-16 | E-MTAB-12559 | biostudies-arrayexpress

RNA-seq analysis of resting and inflamed human neutrophils

Project description:RNA-seq analysis of resting and inflamed human neutrophils

| PRJNA794850 | ENA

RNA-seq analysis of resting and inflamed human neutrophils

Project description:This study includes RNA-seq profiles of neutrophils from healthy donors and patients with inflammatory arthritis.

2022-02-19 | GSE193117 | GEO

Gene expression profile at single cell level of intraepithelial neutrophils (IENs) and lamina propria neutrophils (LPNs) of the colon from dextran sulfate sodium (DSS)-treated mice

Project description:We used single cell RNA sequencing (scRNA-seq) to analyze the transcriptional differences of spatially-separated populations of neutrophils in the inflamed colon.

2025-03-10 | GSE278437 | GEO

Single cell transcriptomic profiling of murine adult healthy gingiva

Project description:Single cell transcriptomic profiling of murine adult healthy gingiva

| PRJNA1111751 | ENA

Transcriptional profiles of pediatric SLE neutrophils and Healthy neutrophils cultured with SLE sera or Interferon

Project description:Mature neutrophis were freshly isolated from blood of pediatric systemic lupus erythematosus (SLE) patients and healthy donors. Illumina microarray was used to assess transcriptional changes between SLE group and Control group. To uderstand further the gene expression difference between SLE and healthy neutrofils, neutrophils from healthy donors were cultured with autologous sera, SLE sera or Interferon and microarray data was used to compare with fresh SLE neutrophils. (Expt 1) Neutrophils from 21 SLE samples (19 patients) and 12 healthy donors were isolated, and extracted RNAs were used generate microarray data. (Expt 2) Neutrophils isolated from 2 healthy children (not used in the first experiment) were cultured with autologous sera (control), Interferon alpha (100U and 1000U), and 4 SLE sera and 6 SLE sera for 6 hours and RNAs were extract for microarray experiment.

2011-03-10 | E-GEOD-27427 | biostudies-arrayexpress

Proteomic characterization of 1,000 human and murine neutrophils freshly isolated from blood and sites of sterile inflammation

Project description:Neutrophils, crucial player in inflammation, present a significant challenge in comprehensive molecular characterization due to limited availability (~1,000 cells) in inflamed sites like steady state infiltration. Prior proteomic studies typically required millions of neutrophils, posing difficulties when dealing with limited samples. This study introduces a ‘low-cell proteome’ workflow for neutrophils, integrating S-trap micro column-based digestion with DIA PASEF to investigate their functional dynamics within individual inflamed site.This method enable protein profiling of 1,000 isoated resting circulatory neutrophils with broad dynamic range in a reproducible manner.Furthermore, we applied this integrated approach to investigate the change in neutrophil proteome under different physiological and pathological conditions, including brain infiltration following stroke in mice and transmigration to the oral cavity post-treatment with tabasco in humans. Our comprehensive proteomic analysis provides insights into the distinct behavioral responses of neutrophils in these inflammatory contexts compared to their circulating counterparts.

2024-10-11 | PXD050682 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data