Project description:In present experiment we evaluated the effects of the utrasonication of winter wheat seeds on seedling growth and development. Effect of treatment on the gene transcription and DNA methylation of seedlings were evaluated.
Project description:In present experiment we evaluated the effects of the utrasonication of winter wheat seeds on seedling growth and development. Effect of treatment on the gene transcription and DNA methylation of seedlings were evaluated.
Project description:Plants of two non-restorer varieties of hexaploid winter wheat (Astoria, Grana) and two restorers ones (Patres and Primépi) were used to identify effective Rf (fertility restorer) genes by next generation sequencing on whole transcriptomes (RNA-seq).
Project description:Wheat is the staple food of over 35% of the world’s population, accounts for 20% of all human calories, and its yield and quality improvement is a focus in the effort to meet new demands from population growth and changing diets. As the complexity of the wheat genome is unravelled, determining how it is used to build the protein machinery of wheat plants is a key next step in explaining detailed aspects of wheat growth and development. The specific functions of wheat organs during vegetative development and the role of metabolism, protein degradation and remobilisation in driving grain production are the foundations of crop performance and have recently become accessible through studies of the wheat proteome. With the aim of creating a resource complementary to current genome sequencing and assembly projects and to aid researchers in the specific analysis and measurement of wheat proteins of interest, we present a large scale, publicly accessible database of identified peptides and proteins derived from the proteome mapping of Triticum aestivum. This current dataset consists of twenty four organ and developmental samples in an online interactive resource allowing the selection, comparison and retrieval of proteomic data with rich biochemical annotation derived from multiple sources. Tissue specific sub-proteomes and ubiquitously expressed markers of the wheat proteome are identified alongside hierarchical assessment of protein functional classes and their presence in different tissues. The impact of wheat’s polyploid genome on proteome analysis and the effect on defining gene specific and protein family relationships is accounted for in the organisation of the data. The dataset will serve as a vehicle to build, refine and deposit confirmed targeted proteomic assays for wheat proteins and protein families to assess function.
Project description:To better understand the regulatory mechanisms of water stress response in wheat, the transcript profiles in roots of two wheat genotypes, namely, drought tolerant 'Luohan No.2' (LH) and drought susceptible 'Chinese Spring' (CS) under water-stress were comparatively analyzed by using the Affymetrix wheat GeneChip®. A total of 3831 transcripts displayed 2-fold or more expression changes, 1593 transcripts were induced compared with 2238 transcripts were repressed, in LH under water-stress; Relatively fewer transcripts were drought responsive in CS, 1404 transcripts were induced and 1493 were repressed. Comparatively, 569 transcripts were commonly induced and 424 transcripts commonly repressed in LH and CS under water-stress. 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories, and 74 transcripts derived from 80 probe sets displayed the change ratios no less than 16 in LH or CS. Several kinds of candidate genes were differentially expressed between the LH and CS, which could be responsible for the difference in drought tolerance of the two genotypes.
Project description:The aim of this study was to identify genetic mechanisms of tolerance to active de-acclimation in winter wheat by comparing four tolerant and four susceptible accessions (cultivars and advanced breeding lines). The plants were kept in controlled conditions. 21 days after sowing, the plants were cold acclimated (3 weeks, 4/2 °C, day/night temperature; photoperiod of 10/14 h). Cold acclimation was followed by 7 days of de-acclimating conditions, mimicking a mid-winter warm spell: 12/5°C, day/night temperature; photoperiod of 10/14 h). The fragments of leaves were sampled in three biological replicates (three different plants of the same accession) in three timepoints: just before cold-acclimation (CA-0), after cold-acclimation (CA-21), and after de-acclimation (DA-28). Total RNA samples were sequenced on Illumina NovaSeq 6000 platform in PE150 bp mode. Differences in expression for a given accession were tested between all time-points, averaging the results for three biological replicates. It has been confirmed that active de-acclimation is not simply the inverse of cold acclimation, and that susceptible forms show significantly more changes in expression as a result of de-acclimation than tolerant forms. This provides further support for the hypothesis that tolerance to de-acclimation consists mainly of the absence or weakest response to temperature rise. Among the genes whose expression was significantly altered as a result of de-acclimation, a significant group were genes related to the defence response to stress. In wheat particularly plant hormone related genes, and plant hormones, seem to be playing a crucial role in the response to active de-acclimation, especially ABA and SA. Our results also suggest that in wheat the period of cold-acclimation might be decisive in the later active de-acclimation tolerance.