Project description:To examine the role of hepatpcyte growth factor activator inhibitor type 1 (HAI-1) in cancer, we analyzed effect of HAI-1 silencing on gene expression profiles of human oral squamous cell carcinoma cell line, SAS. We used short hairpin RNA (shRNA) directed against HAI-1 mRNA. We constructed retroviral vectors which showed stable and significant silencing effects on HAI-1 genes of SAS. Microarray data of the expression profiles of duplicated experiments of HAI-1-knockdown SAS with that from the control cell are shown.
Project description:Complement-dependent cytotoxicity (CDC) is an important effector function of various therapeutic antibodies. Cancer cells often develop resistance to CDC, mainly due to external factors. In this study, using models of diffuse large B-cell lymphoma (DLBCL), we compared gene expression levels between the parental U2932 and CDC-resistant U2932 DLBCL cell lines, as well as gene expression differences between highly CDC-sensitive and low CDC-sensitive DLBCL patient samples. Hereby, we aimed to identify intrinsic regulators of CDC.
