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Transcriptional effects of YAP/TAZ knockout in primary mouse dermal fibroblasts

ABSTRACT: Transcriptional effects of YAP/TAZ knockout in primary mouse dermal fibroblasts

PROVIDER: PRJNA695286 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13565847.fastq.gz	Fastqsanger.gz
	SRR13565848.fastq.gz	Fastqsanger.gz
	SRR13565849.fastq.gz	Fastqsanger.gz
	SRR13565850.fastq.gz	Fastqsanger.gz
	SRR13565851.fastq.gz	Fastqsanger.gz

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Transcriptional effects of YAP/TAZ knockout in primary mouse dermal fibroblasts

Project description:We compared the transcriptomes of control and YAP/TAZ knockout fibroblasts, and the latter in the presence or absence of a STING inhibitor

2022-06-20 | GSE165665 | GEO

Transcriptional effects of YAP/TAZ knockdown in human fibroblasts

Project description:We compared the transcriptomes of control and YAP/TAZ-depleted fibroblasts, in the presence or absence of cGAS or STING depletion

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Increased expression of yes-associated protein/YAP and transcriptional coactivator with PDZ-binding motif/TAZ activates intestinal fibroblasts to promote intestinal obstruction in Crohn’s disease

Project description:The results of this study indicate that the expression of YAP/TAZ was significantly upregulated in stenotic fibroblasts, which was associated with the YAP/TAZ target gene signature. YAP/TAZ knockdown suppressed the activation of intestinal fibroblasts. In intestinal fibroblasts, YAP/TAZ were activated by the Rho-ROCK1 signalling pathway. High YAP/TAZ expression was positively correlated with ROCK1 expression, which is a prognostic marker for intestinal obstruction in CD patients.

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Yap and Taz regulate retinal pigment epithelial cell fate

Project description:The optic vesicle comprises a pool of bi-potential progenitor cells from which the retinal pigment epithelium (RPE) and neural retina fates segregate during ocular morphogenesis. Several transcription factors and signaling pathways have been shown to be important for RPE maintenance and differentiation, but an understanding of the initial fate specification and determination of this ocular cell type is lacking. We show that Yap/Taz-Tead activity is necessary and sufficient for optic vesicle progenitors to adopt RPE identity in zebrafish. A Teadresponsive transgene is expressed within the domain of the optic cup from which RPE arises, and Yap immunoreactivity localizes to the nuclei of prospective RPE cells. yap (yap1) mutants lack a subset of RPE cells and/or exhibit coloboma. Loss of RPE in yap mutants is exacerbated in combination with taz (wwtr1) mutant alleles such that, when Yap and Taz are both absent, optic vesicle progenitor cells completely lose their ability to form RPE. The mechanism of Yap dependent RPE cell type determination is reliant on both nuclear localization of Yap and interaction with a Tead co-factor. In contrast to loss of Yap and Taz, overexpression of either protein within optic vesicle progenitors leads to ectopic pigmentation in a dosagedependent manner. Overall, this study identifies Yap and Taz as key early regulators of RPE genesis and provides a mechanistic framework for understanding the congenital ocular defects of Sveinsson’s chorioretinal atrophy and congenital retinal coloboma. 60 pooled eyes from 36 hpf wild type or vsx2:Gal4/dsRed:14xUAS:YapS87A embryos were pooled for one sample. Three wild type and three vsx2:Gal4/dsRed:14xUAS:YapS87A pools were analyzed for RNA.

2015-08-06 | E-GEOD-71681 | biostudies-arrayexpress

Transcriptional effects of YAP/TAZ knockdown in fibroblasts

Project description:Transcriptional effects of YAP/TAZ knockdown in fibroblasts

| PRJNA695285 | ENA

YAP/TAZ activity in PDGFRa-expressing alveolar fibroblasts modulates lung fibroblast-epithelial cell crosstalk through Wnt4 [scRNA-seq]

Project description:The Hippo signaling pathway, mediated by its transcriptional effectors YAP and TAZ, play vital roles in maintaining lung homeostasis and facilitating injury repair. While the roles of the Hippo pathway in epithelial cells are well-established, its regulatory effects on lung fibroblasts remain less understood. Here, we engineered a novel mouse allele to allow inducible knockdown of YAP and TAZ, and show that fibroblast-specific knockdown enhances the ability of PDGFRa+ alveolar fibroblasts to support organoids derived from alveolar epithelial stem cells in vitro. Single-cell multiomic profiling revealed changes in fibroblast subpopulations, including the emergence of an Mmp9+ cluster containing Wnt4+ cells. Analyses demonstrated shifts in the epigenomic landscape leading to varied enrichment of transcription factor motifs across both fibroblasts and epithelial cells in response to targeted suppression of YAP/TAZ in fibroblasts. Further computational analyses identified an increase in epithelial Wnt signaling which was confirmed by in vivo studies. We found that Wnt4 expression was increased in PDGFRa-lineage+ fibroblasts and enhanced proliferation of SPC+ AT2 cells following fibroblast-specific YAP/TAZ knockdown. These results shed new light on the mechanistic role of YAP/TAZ in PDGFRa+ alveolar fibroblasts in supporting AT2 cell maintenance and proliferation via Wnt4 secretion.

2025-04-10 | GSE291641 | GEO

YAP/TAZ activity in PDGFRa-expressing alveolar fibroblasts modulates lung fibroblast-epithelial cell crosstalk through Wnt4 [scATAC-seq]

2025-04-10 | GSE291640 | GEO

Transcriptional effects of YAP/TAZ knockout in mouse gliomaspheres

Project description:We compared the transcriptomes of control and YAP/TAZ knockout gliomaspheres derived from mouse newborn astrocytes transformed with KRAS/shp53. We wanted to evaluate the effects of YAP/TAZ depletion on gliomaspheres transcriptional program.

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The effect of YAP/TAZ knockdown on the intestinal epithelium

Project description:The Hippo pathway plays a crucial in organ size control during development and tissue homeostasis in adult life. To examine a role for Hippo signaling in the intestinal epithelium, we analyzed gene expression patterns in the mouse intestinal epithelilum transfected with siRNAs or expression plasmids for shRNAs targeting the Hippo pathway effectors, YAP and TAZ. We performed two independent series of experiments (siGFP (n=3) vs siYAP/siTAZ (n=3), and shLacZ (n=1) vs shYAP/shTAZ (n=1)). Control siRNA (siGFP), YAP/TAZ siRNAs, or expression plasmids for control shRNA (shLacZ) or YAP/TAZ shRNAs were introduced into the mouse intestinal epithelium by the newly-developed in vivo transfection method. Four days after transfection, intestinal epithelial cells were isolated from the tissues and total RNA was extracted.

2014-07-18 | E-GEOD-45155 | biostudies-arrayexpress

Combined control of the fibroblast contractile program by YAP and TAZ

Project description:YAP and TAZ are transcription cofactors implicated in the contractile and pro-fibrotic activation of fibroblasts. Fibroblast contractile function is important in alveologenesis, as well as in lung wound healing and fibrosis. As paralogs, YAP and TAZ may have independent or redundant roles in regulating transcriptional programs and contractile function. Using IMR-90 lung fibroblasts, microarray analysis and traction microscopy we tested whether independent YAP or TAZ knockdown alone was sufficient to limit transcriptional activation and contraction in vitro.

2021-06-01 | GSE175853 | GEO

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