Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [4C-seq]

ABSTRACT: BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [4C-seq]

PROVIDER: PRJNA695528 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13571564.fastq.gz	Fastqsanger.gz
	SRR13571565.fastq.gz	Fastqsanger.gz
	SRR13571566.fastq.gz	Fastqsanger.gz
	SRR13571567.fastq.gz	Fastqsanger.gz
	SRR13571568.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [4C-seq]

Project description:The cystic fibrosis transmembrane conductance regulator (CFTR) gene lies within a TAD in which multiple cis-regulatory elements (CREs) and transcription factors (TFs) regulate its cell-specific expression. The CREs are recruited to the gene promoter by a looping mechanism that depends upon both architectural proteins and specific TFs. An siRNA screen to identify TFs coordinating CFTR expression in airway epithelial cells suggested an activating role for BTB Domain and CNC Homolog 1 (BACH1). BACH1 is a ubiquitous master regulator of the cellular response to oxidative stress. Here we show that BACH1 may have a dual effect on CFTR expression by direct occupancy of CREs at physiological oxygen (~8%), while indirectly modulating expression under conditions of oxidative stress. Hence BACH1, can activate or repress the same gene, to fine tune expression in response to environmental cues such as cell stress. Furthermore, our 4C-seq data suggest that BACH1 can also directly regulate CFTR gene expression by modulating locus architecture through occupancy at known enhancers and structural elements, and depletion of BACH1 alters the higher order chromatin structure.

2021-11-17 | GSE165716 | GEO

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [ChIP-seq]

2021-11-17 | GSE165717 | GEO

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [RNA-seq]

2021-11-17 | GSE165718 | GEO

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression

Project description:BACH1, the master regulator of oxidative stress, modulates CFTR gene expression

| PRJNA695525 | ENA

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [RNA-seq]

Project description:BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [RNA-seq]

| PRJNA695530 | ENA

BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [ChIP-seq]

Project description:BACH1, the master regulator of oxidative stress, modulates CFTR gene expression [ChIP-seq]

| PRJNA695529 | ENA

BACH1 as a master regulator gene in vascular cells during aging

Project description:We identify transcription factor BACH1 as a master regulator in vascular cells during aging. BACH1 is upregulated in the aorta of old mice. We find BACH1 is located on open chromatin and BACH1 binds to CDKN1A gene enhancer and activates its transcription in endothelial cells. Finally, BACH1 aggravates endothelial cell senescence under oxidative stress. Thus, these findings demonstrate a crucial regulatory role of BACH1 in vascular aging.

2024-09-15 | GSE185210 | GEO

BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression.

Project description:The cystic fibrosis transmembrane conductance regulator (CFTR) gene lies within a topologically associated domain (TAD) in which multiple cis-regulatory elements (CREs) and transcription factors (TFs) regulate its cell-specific expression. The CREs are recruited to the gene promoter by a looping mechanism that depends upon both architectural proteins and specific TFs. An siRNA screen to identify TFs coordinating CFTR expression in airway epithelial cells suggested an activating role for BTB domain and CNC homolog 1 (BACH1). BACH1 is a ubiquitous master regulator of the cellular response to oxidative stress. Here, we show that BACH1 may have a dual effect on CFTR expression by direct occupancy of CREs at physiological oxygen (∼8%), while indirectly modulating expression under conditions of oxidative stress. Hence BACH1, can activate or repress the same gene, to fine tune expression in response to environmental cues such as cell stress. Furthermore, our 4C-seq data suggest that BACH1 can also directly regulate CFTR gene expression by modulating locus architecture through occupancy at known enhancers and structural elements, and depletion of BACH1 alters the higher order chromatin structure.

| S-EPMC8589331 | biostudies-literature

New insights into the cis-regulation of the CFTR gene in pancreatic cells [4C-seq]

Project description:To understand the role of 3D chromatin architecture in establishing tissue-specific expression of the CFTR gene, we mapped chromatin interactions using Circular Chromosome Conformation Capture (4C) and epigenomic data such as H3K27ac and Assay for Transposase-Accessible Chromatin (ATAC-seq) on Capan-1 pancreatic cells.

2025-01-27 | GSE284199 | GEO

Dual BACH1 regulation by complementary SCF-type E3 ligases

Project description:BACH1, FBXO22, FBXL17, oxidative stress response, E3, Cullin-RING ligases, F-box, cysteine modification, S-nitrosylation, heme

2024-12-03 | PXD051074 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data