Project description:In this study, early infection by F. graminearum was characterized in a doubled-haploid population derived from the cross of the moderately FHB resistant wheat genotypes Wuhan 1 and Nyubai. Three significant QTL were identified: 1AL was associated with reduced deoxynivalenol levels, and 4BS and 5A were associated with reduced F. graminearum levels at 2 days after infection. The early resistance alleles for 1AL and 4BS were inherited from Wuhan 1, and those for the 5A QTL were inherited from Nyubai. The LOD support interval for the 5A QTL was in the same region as FHB5, a QTL previously associated with field resistance to FHB. Cis and trans eQTL were identified using RNA sequencing data from infected head samples. In total, 240 cis eQTL and 17,401 trans eQTL were identified within LOD support intervals for the three QTL. Hotspots for trans eQTL were identified within LOD support intervals for the 1AL and 4BS QTL. Differential gene expression analysis revealed twenty genes with cis eQTL within one of the 3 QTL support intervals, that had higher expression associated with FHB early resistance, and 17 genes with higher expression associated with FHB early susceptibility. Among those, genes with a cis eQTL peak coinciding with QTL peaks were enriched in functions related to signaling and protein-protein interaction, including NBS-LRR, BAH, LRR/F-box and TPR domain-containing proteins. Several genes located within one of the three QTL support intervals also had a trans eQTL with a peak at the same position as QTL peaks on other chromosomes, suggesting interactions between the QTL.
Project description:A novol fruit weight QTL fw2.3 was identified in tomato populaiton developed from a cross between two semi-domesticated SLC accession: BGV006768 and BGV007931. The effect of fw2.3 was observed in ovaries at anthesis stage, before fruit development. So young infloresences from F3 progenies with contrasting genotype of fw2.3 were used in RNA-seq experiment. Differentially expressed genes in the fw2.3 interval may help us to locate causal genes of this fruit weight QTL.
Project description:More than 20 years ago, the FW2.2 gene was the first cloned gene underlying a Quantitative Trait Locus (QTL) governing fruit size/weight in tomato. However, despite this discovery, the molecular mechanisms by which FW2.2 acts as a negative regulator of cell divisions during fruit growth remain undeciphered. Here we performed a co-immunoprecipitation experiment on protein extract from pericarp of fruits 10 days after anthesis from wild-type and 35S::FW2.2-YFP plants in order to identify interacting proteins of FW2.2.
Project description:Tomato is a major global crop however its production is limited by Botrytis cinerea. Due to the toxicity of post-harvest pesticide application, alternative control methods such as priming are being investigated. Plants were treated with β-aminobutyric (BABA) at two developmental stages and resistance against B. cinerea was tested in fruit tissue and in progenies. DNA methylation and RNA sequencing were conducted to characterise the (epi)genetic changes associated with long-lasting resistance. Grafting experiments were done to assess the systemic nature of this signal, which was further characterised by small RNA (sRNA) sequencing of scions. Only BABA-treated seedlings displayed induced resistance (IR). DNA methylation analysis revealed seedling-specific changes, which occurred in the context of lower basal methylation. BABA-IR was found to be transmissible from primed rootstock to grafted unprimed scions. In these scions, we identified a subset of mobile 24 nt sRNAs associated with primed genes to infection in fruit. Our results demonstrate the functional association of a systemic signal with long lasting IR and priming. Through integrated omics approaches we have identified markers of long-lasting priming in tomato fruit which could also serve as targets for durable resistance in other crops.
Project description:We used mRNA sequencing to study the effects of piperonylic acid treatment on the transcriptome of tomato plants (cv. Moneymaker) to gain insights in the molecular basis of piperonylic acid-induced disease resistance
Project description:4plex_tomato_2013_03 - 4plex_tomato_2013_03 - What are the genes implied in drought resistance in tomato ? - 2 strains of tomato (Cervil, a cherry tomato, and Levovil, an large fruited tomato) were cultivated under normal hydric conditions and drought stress. Young leaves were gathered 3 1/2 months after sawing for analysis.