Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Differential circRNA expression in stored red blood cells

PROVIDER: PRJNA698384 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13649554_1.fastq.gz	Fastqsanger.gz
	SRR13649554_2.fastq.gz	Fastqsanger.gz
	SRR13649555_1.fastq.gz	Fastqsanger.gz
	SRR13649555_2.fastq.gz	Fastqsanger.gz
	SRR13649556_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Project description:Differential circRNA expression profile in stored red blood cells

| PRJNA698240 | ENA

Abundant Circular RNA expression in terminal erythropoiesis and megakaryopoiesis shows limited evidence for translation or circRNA-mediated translation regulation [Ribo-seq]

Project description:Erythrocytes and platelets have high production rates, with 1012 cells released daily into the blood stream. This output from bone-marrow residing hematopoietic stem cells is tightly regulated by transcription factors and epigenetic modifications. Whether and how non-coding RNAs such as circular RNAs (circRNAs) contribute to the differentiation and/or identity of hematopoietic cells is to date not well understood. We recently published a circRNA expression map of hematopoietic cells, which showed that erythrocytes and platelets contain the highest levels and numbers of circRNAs. Whether and how circRNA expression alters during differentiation of erythrocytes and platelet precursors is however not known. Therefore, we here provide the first detailed and comprehensive analysis of circRNA expression during red blood cell and megakaryocyte differentiation. CircRNA expression significantly increased during erythroid precursor differentiation into red blood cells, and in differentiating megakaryocytes, in particular upon enucleation. Many functions have been attributed to circRNAs. To dissect their possible function in hematopoietic differentiation, we first focused on translation regulation. We compared circRNA and mRNA expression to ribosomal foot printing data, and found that only 20 (2.6%) circRNAs associated with translation regulation of their mRNA counterparts. We also identified thousands of putative open reading frames in circRNAs, suggesting that circRNAs may also encode proteins. However, deep ribosome-footprinting sequencing data and in-depth mass spectrometry data analysis provided little evidence for translation of endogenously expressed circRNAs in erythroblasts, megakaryocytes and platelets. In conclusion, circRNAs in platelets and red blood cells are highly abundant and alter their expression profile during differentiation, yet their contribution to regulate cellular processes remains enigmatic.

2021-03-04 | GSE159578 | GEO

Abundant Circular RNA expression in terminal erythropoiesis and megakaryopoiesis shows limited evidence for translation or circRNA-mediated translation regulation [RNA-seq]

2021-03-04 | GSE159577 | GEO

Pre-transfusion storage alters the proteostasis function and capacity to circulate of a morphologically- and metabolically-altered red cell subpopulation, but preserves other red cells

Project description:This study aimed to provide a characterization of morphologically-altered red blood cells (RBCs) and to compare their properties to those of long-stored morphologically normal RBCs and to short-stored RBCs. For proteomics experiments, RBC concentrates stored in blood bank conditions were submitted to a CFSE staining protocol that allows specific sorting of morphologically-altered (CFSEhigh) and normal (CFSElow) RBC subpopulations. Proteome of erythrocytes and ghosts were analyzed at day 3-10 (short-stored, CFSElow RBC subpopulation) and day 40-44 (long-stored, CFSEhigh and CFSElow RBC subpopulations) of storage by a label free quantification approach.

2025-04-02 | PXD049411 | Pride

ACUTE BLEEDING AND STORED RED BLOOD CELLS TRANSFUSION ATTENUATES LIVER REGENERATION FOLLOWING PARTIAL HEPATECTOMY

Project description:ACUTE BLEEDING AND STORED RED BLOOD CELLS TRANSFUSION ATTENUATES LIVER REGENERATION FOLLOWING PARTIAL HEPATECTOMY

| PRJNA896192 | ENA

Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis

Project description:In order to identify new biomarkers for the diagnosis of rheumatoid arthritis, we used circRNA microarray technology to screen the differential expression of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis. We identified a total of 399 differentially expressed circRNAs in RA patients and healthy controls, of which 149 circRNAs were significantly up-regulated in RA patients and 250 were down-regulated. Among them, hsa_circRNA_101328 may be a potential biomarker for the diagnosis of RA.

2021-11-30 | GSE189338 | GEO

Project description:circRNA identification and differential expression

| PRJNA762602 | ENA

Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion

Project description:We conducted an animal experiment and used total RNA sequencing (RNA-Seq) to identify novel RNA markers to detect autologous blood transfusion (ABT) doping within red blood cells (RBCs) as a pilot study before human trials. The total RNA-Seq and bioinformatics were performed using rat RBCs that were stored 10- and 20-day periods with citrate–phosphate–dextrose solution with adenine (CPDA) at 4°C In the results, we identified three significant genes whose expression levels were drastically decreased according to the storage period. Our novel insights may allow future studies to develop a test method for ABT doping.

2022-07-14 | GSE205806 | GEO

Circular RNA expression profile in human bladder cancer cell line and human urothelial immortalized cell line

Project description:To further explore the differential expression profile of circRNA in human bladder cancer, we have employed circRNA microarray expression profiling as a discovery platform to identify potential differential expression profile of circRNA between human bladder cancer cell (UM-UC-3 cell) and urothelial immortalized cell (SV-HUC-1 cell). Results showed a large number of differentially expressed circRNAs. In this study, we screened the eight circRNAs that were most significantly downregulated in the circRNA differential expression profile of bladder cancer cells, designed primers across the splice site, and detected them using qPCR. We found that hsa_circ_0047253 (circLAMA3) was most significantly decreased in the bladder cancer cells, then its biological function and action mechanism have been further explored in this study.

2022-02-08 | GSE159239 | GEO

Cytoskeleton

Project description:Human red blood cells

| PAe000044 | PeptideAtlas