Project description:To determine the affect of CRISPR interference-mediated silencing of glycosylation in Burkholderia diffusa harbouring a pglL (WL85_RS02125)-targeted guide, and a nontarget guide using Data Independent Acquisition
Project description:To determine whether CRISPR interference can be used to silence the expression of pglL in Burkholderia diffusa and therefore modulate glycosylation
Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia diffusa through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment
Project description:To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia diffusa through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment – loading control pre-enrichment
Project description:This genome-scale metabolic model (GEM) of Corynebacterium tuberculostearicum strain DSM 44922 (Taxon ID 38304) was initially built with CarveMe version 1.5.1 based on the genome assembly with NCBI accession GCF_013408445.1 and then underwent a series of careful semi-automatic and manual curation. It is the first model curated using the Python tool MCC for mass and charge curation.