Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Experimental vaccination and infection of eastern hellbender salamanders with Batrachochytrium dendrobatidis

PROVIDER: PRJNA700070 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13660600_1.fastq.gz	Fastqsanger.gz
	SRR13660600_2.fastq.gz	Fastqsanger.gz
	SRR13660601_1.fastq.gz	Fastqsanger.gz
	SRR13660601_2.fastq.gz	Fastqsanger.gz
	SRR13660530_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 292

Similar Datasets

Transcription profiling of Xenopus tropicalis in response to infection by the chytrid Batrachochytrium dendrobatidis

Project description:Chytridiomycosis is an emerging infectious disease of amphibians caused by the chytrid Batrachochytrium dendrobatidis (Bd). The disease has been associated with global amphibian declines and is driving the species in the wild to extinction. Using DNA microarray technology we have analysed transcriptional changes in Xenopus tropicalis during the course (7 and 42 days) of infection by Bd under warm (26oC) and cold (18oC) temperatures.

2009-10-22 | E-MEXP-1936 | biostudies-arrayexpress

Common frog (Rana temporaria) transcriptomes following Exposure to Ranavirus and Batrachochytrium dendrobatidis

Project description:This experiment examined the transcriptional response of juvenile amphibian hosts (common frog, Rana temporaria) to two important amphibian pathogens: Batrachochytrium dendrobatidis (Bd) and Ranavirus. Common frogs are non-model organisms which do not have a reference genome.

2015-07-10 | E-MTAB-3632 | biostudies-arrayexpress

Boreal toad transcriptomic response to Bd infection

Project description:Boreal toads (Anaxyrus boreas boreas) of the Southern Rocky Mountain population are declining due to the introduction of the chytrid fungus Batrachochytrium dendrobatidis (Bd). Boreal toads in Colorado are generally susceptible to Bd infection, but some Bd-tolerant populations persist in parts of the Southern Rocky Mountain and broader Eastern boreal toad population. We conducted a Bd challenge with lab-reared sibling toads from Bd-susceptible Colorado and purportedly Bd-tolerant Utah populations and report on transcriptomic responses to Bd during late infection in skin and liver tissue. Fewer immune genes were expressed in response to Bd in Colorado toads, but with greater upregulation compared to Utah toads, indicating a dysregulated immune response. Signatures of Bd-tolerance in Utah toads included more moderate upregulation in immune gene expression and a significantly enriched suite of gene functions related to innate and adaptive immune responses. Our transcriptomic results support the notion that Utah toads are tolerant to Bd, rather than resistant, carrying Bd loads similar to Colorado yet having a unique transcriptomic profile and presenting minimal clinical signs of chytridiomycosis. We conclude that closely related populations have divergent transcriptomic responses to Bd with a dysregulated immune response in Bd-susceptible toads.

2023-10-04 | GSE244617 | GEO

Batrachochytrium dendrobatidis strain:Section Line

Project description:Batrachochytrium dendrobatidis Genome resequencing

| PRJNA265883 | ENA

Project description:Microbial response to experimental Batrachochytrium salamandrivorans and B. dendrobatidis introduction.

| PRJNA897848 | ENA

Transcriptomic Analysis of the Serratia marcescens Response to Batrachochytrium dendrobatidis

Project description:Global amphibian declines and extinction events are currently occurring at an unprecedented rate. While various factors are influencing these declines, one factor that is readily identifiable is disease. Specifically, the fungal pathogen Batrachochytrium dendrobatidis is thought to play a major role in amphibian declines in tropical and neotropical regions of the globe. While the effects of this chytrid fungus have been shown to be devastating, certain individuals and relict populations have shown resistance. This resistance has been attributed in part to the cutaneous microbiome. Many identified bacterial species that make up the microbiome have shown anti-B. dendrobatidis activity in vitro. One bacteria that is commonly associated as being a member of the amphibian microbiome across amphibian species and shows such anti-B. dendrobatidis activity is Serratia marcescens. Here, we look at transcriptomic shifts in gene expression of S. marcescens (high homology to strain WW4) in response to both live and heat-killed B. dendrobatidis.

2016-07-11 | GSE84057 | GEO

Substrate-specific gene expression in the amphibian chytrid pathogen

Project description:Determining the mechanisms of host-pathogen interaction is critical for understanding and mitigating infectious disease. Mechanisms of fungal pathogenicity are of particular interest given the recent outbreaks of fungal diseases in wildlife populations. Our study focuses on Batrachochytrium dendrobatidis (Bd), the chytrid pathogen responsible for amphibian declines around the world. Previous studies have hypothesized a role for several specific families of secreted proteases as pathogenicity factors in Bd, but the expression of these genes has only been evaluated in laboratory growth conditions. Here we conduct a genome-wide study of Bd gene expression under two different nutrient conditions. We compare Bd gene expression profiles in laboratory growth media and in host tissue (i.e., frog skin). A large proportion of genes in the Bd genome show increased expression when grown in host tissue, indicating the importance of studying pathogens in their infection environment. A number of gene classes show particularly high levels of expression in host tissue, including three families of secreted proteases (metallo-, serine- and aspartyl-proteases), adhesion genes, lipase-3 encoding genes, and a group of phylogenetically unusual crinkler-like effectors. We discuss the roles of these different genes as putative pathogenicity factors and discuss what they can teach us about Bd's metabolic targets, host invasion, and pathogenesis. One 12-plex chip was analyzed from 12 RNA samples extracted from Batrachochytrium dendrobatidis grown in 2 substrates. Six biological replicates were used for each substrate - sterile frog skin and tryptone nutrient broth. The same dye, Cy5, was used for all samples.

2012-11-01 | E-GEOD-37135 | biostudies-arrayexpress

Batrachochytrium dendrobatidis JEL423

Project description:Batrachochytrium dendrobatidis JEL423 Genome sequencing

| PRJNA1109278 | ENA

Batrachochytrium dendrobatidis JAM81

Project description:Batrachochytrium dendrobatidis JAM81 genome sequencing project

| PRJNA41157 | ENA

Batrachochytrium dendrobatidis strain:multiple strains

Project description:Multi-isolate project for Batrachochytrium dendrobatidis

| PRJNA63423 | ENA