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RNAseq analysis of integrin β3-mediated responses to in vitro docetaxel in PyMT-BO1 breast cancer cells

ABSTRACT: RNAseq analysis of integrin β3-mediated responses to in vitro docetaxel in PyMT-BO1 breast cancer cells

PROVIDER: PRJNA700430 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13648627.fastq.gz	Fastqsanger.gz
	SRR13648628.fastq.gz	Fastqsanger.gz
	SRR13648629.fastq.gz	Fastqsanger.gz
	SRR13648630.fastq.gz	Fastqsanger.gz
	SRR13648631.fastq.gz	Fastqsanger.gz

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RNAseq analysis of integrin β3-mediated responses to in vitro docetaxel in PyMT-BO1 breast cancer cells

Project description:We sought to transcriptomically characterize the effect of integrin β3 expression on breast cancer cell responses to the microtubule-inhibiting chemotherapeutic agent docetaxel. Experiments were performed in PyMT-BO1 murine breast cancer cells with CRISPR mediated deletion of the Itgb3 gene. Cells were subsequently retrovirally rescued using either an empty vector construct (pMx), a functional human integrin β3 construct (hβ3), or a signaling-deficient integrin β3 mutant.

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