Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:In this research, Pseudomonas aeruginosa underwent evolution in the supernatant of either S. aureus (SA) or K. pneumoniae (KP). Through this evolution process, we enriched a sufficient number of bacterial cells along with their corresponding proteins. Following 15 cultivation cycles, notable changes were observed in the phenotypes of P. aeruginosa, with a series of mutations identified through whole-genome sequencing (WGS). Proteomics analysis unveiled that the supernatant-evolved mutants displayed distinct regulation patterns in crucial pathways (including the type VI secretion system, biofilm formation, phenazine biosynthesis, translation, beta-lactam resistance, and O-antigen biosynthesis) in comparison to both the ancestral strain and the unmodified medium-evolved (UmMd-evolved) strain.
Project description:To gain insights into the mechanisms by which RC301 compensates for the deficiency in the NPR-1 controlled immune and behavioral responses of strain DA650, we determine the whole-genome expression profile of these two strains upon exposure to Pseudomonas aeruginosa strain PA14
Project description:Drosophila melanogaster is a validated eukaryotic model for immunity-concerned studies in the post-genomic era. In the present study we performed oral experimental infection of D. melanogaster with Pseudomonas aeruginosa (strain ATCC27853). By using a whole genome microarray approach, we intended to identify significant alterations in the expression profile of relevant genes amenable to qualify as new models for the investigation of specific host-parasite interactions.