Ontology highlight
ABSTRACT:
PROVIDER: PRJNA701621 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Global studies of microbial diversity
| PRJNA1008400 | ENA
Project description:Infections of burn wounds, especially those caused by Pseudomonas aeruginosa, could trigger sepsis or septic shock, which is the main cause of death after burn injury. Compared with traditional saline-wet-to-dry dressings, negative pressure wound therapy (NPWT) is more effective for the prevention and treatment of wound infections. However, the mechanism by which NPWT controls infection and accelerates wound healing remains unclear. Accordingly, in this study, the molecular mechanisms underlying the effects of NPWT were explored using a murine model of P. aeruginosa-infected burn wounds. NPWT significantly reduced P. aeruginosa levels in wounds, enhanced blood flow, and promoted wound healing. Additionally, NPWT markedly alleviated wound inflammation and increased the expression of wound healing–related molecules. Recent evidence points to a role of circular RNAs (circRNAs) in wound healing; hence, whole-transcriptome sequencing of wound tissues from NPWT and control groups was performed to evaluate circRNA expression profiles.
2022-01-14 | GSE193428 | GEO
Project description:Full thickness and deep partial thickness burn injuries heal by scarring. There are several mechanisms thought to be essential for the development of burn scars, but a challenge to studying the skin response to burn injury is that there are few animal models of burn scarring that are either clinically similar to human burn scars or are practical for most investigators to use. The purpose of this study was to examine the changes in RNA expression in human skin to burn injury. This was done by comparing pre-injury tissue from otherwise healthy adults undergoing aesthetic scarification created by branding with a hot metal object to serial samples of untreated wounds in the same subjects.
2014-01-22 | E-MTAB-1323 | biostudies-arrayexpress
Project description:The goal of this study was to use RNA sequencing to identify novel genes involved in wound healing and scar formation in human burn wounds and scars. RNA was isolated from 108 human samples comprising of uninjured skin (n=26), acute burn wounds (n=54), and hypertrophic scars (HTS) (n=30). Genes with at least 1.5-fold change and a p-value less than 0.05 with a false discovery rate of 0.05 were considered differentially expressed. Samples were sequenced using Illumina Hi-Seq sequencers, and pathway/Gene Ontology (GO) Enrichment analysis was conducted using iPathwayGuide. Comparing wounds to uninjured skin, we found 9,311 differentially expressed genes, accounting for 1,017 GO terms. Comparing HTS to uninjured skin, we found 7,299 differentially expressed genes, accounting for 1,022 GO terms. By analyzing the whole transcriptome, we characterized dominant genes expressed temporally in wounds and scars. This study reveals the complexity of these processes and helps to bring novel genes to light.
2024-06-03 | GSE178411 | GEO
Project description:Burn wound blister fluid is a valuable matrix for understanding the biological pathways associated with burn injury. In this study, 152 blister fluid samples collected from paediatric burn wounds at two different hospitals were analysed using mass spectrometry proteomic techniques. The protein abundance profile at different days post-burn indicated that there were more proteins associated with cellular damage/repair in the first 24 hours, whereas after this point there were more proteins associated with antimicrobial defence and inflammation. The inflammatory proteins persisted at a high level in the blister fluid for more than 7 days. This may indicate that removal of burn blisters prior to two days post-burn is optimal to prevent excessive or prolonged inflammation in the wound environment. Additionally, many proteins associated with the neutrophil extracellular trap (NET) pathway were increased post-burn, further implicating NETs in the post-burn inflammatory response. NET inhibitors may therefore be a potential treatment to reduce post-burn inflammation and coagulation pathology and enhance burn wound healing outcomes.
2024-03-04 | PXD050003 | Pride