Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 17 h

ABSTRACT: The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 17 h

PROVIDER: PRJNA701640 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 21 h

Project description:The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 21 h

| PRJNA701634 | ENA

HCV Hypo- vs. Hyper-Phosphorylated NS5A Interactome

Project description:Chronic hepatitis C virus (HCV) infection is a leading cause of liver cancer. HCV propagation and oncogenicity depend in part on the phosphorylation states of its non-structural protein 5A (NS5A); however, little is known about how hypo- or hyper-phosphorylated NS5A functions. Here, we segregated hypo- from hyper-phosphorylated NS5A in HCV-infected Huh7.5.1 cells with two custom-made specific antibodies and differentiated their interacting proteins with dimethyl labeling-based quantitative proteomics. Bioinformatics analysis revealed that hyper-phosphorylated NS5A preferentially binds the polymerase II-associated factor 1 complex known to alter host gene expression involved in cancer progression. In contrast, hypo-phosphorylated NS5A binds proteins involved in host antiviral response. Moreover, we found that the hypo-phosphorylated NS5A binds DNA-dependent protein kinase catalytic subunit (DNA-PKcs) predicted to phosphorylate NS5A at serine 232, a key amino acid that governs NS5A transition from hypo- to hyper-phosphorylation state. Inhibition of DNA-PKcs with an inhibitor or via gene-specific knockdown significantly reduced serine 232 phosphorylation and NS5A hyper-phosphorylation. Collectively, we have identified a protein kinase that regulates a delicate balance of NS5A between hypo- and hyper-phosphorylation states respectively involved in host antiviral responses and liver cancer progression.

2019-06-17 | PXD012767 | Pride

Tissue-specific gene signatures of gilthead sea bream (Sparus aurata L.) after hyper- and hypo-osmotic challenges

Project description:Euryhaline teleosts can live in a wide range of environmental salinities. Transcriptomic analysis in important osmoregulatory and non-osmoregulatory tissues (liver, gills and hypothalamus) by means of a specific oligo-microarray revealed new aspects related to the osmoregulatory processes mediated by different canonical pathways after hypo- or hyper-omotic challenges in gilthead sea bream (Sparus aurata) juveniles. Liver was the most responsive tissue to salinity changes. In addition, around 24 %, 8 % and 12 % of the total genes differentially expressed were affected and regulated in the same way (up- or down-regulated) by both hyper- and hypo-osmotic challenges in liver, gills and hypothalamus, respectively. In liver and gills, functional analysis of the differentially expressed genes established 2 major clusters of canonical pathways related to i) Energy Metabolism and ii) Oxidative Stress: Damage and Repair, whereas for hypothalamus only a main clusters linked to Oxidative Stress: Damage and Repair was identified. Further, this common cluster presents different sub-nodes in all analyzed tissues, with a clear dualism between liver and gills in those pathways involved in nitrogenous turnover, as well as between gills and hypothalamus in cell stress processes. In addition, several sub-nodes related to cell and tissue architecture was only found in hypothalamus. Moreover, only genes involved in xenobiotic metabolism signaling canonical pathway were differentially regulated by both hypo- and/or hyper-salinity transfer in the three tissues considered. Our results indicated that hepatic, branchial and hypothalamic transcriptome of the sea bream clearly responded after 7 days in different environmental salinities, when mainly metabolic and different cell functions are activated to reach homeostasis, or even return to their basal levels.

2017-02-10 | GSE73872 | GEO

Systematic Pan-cancer Functional Inference and Validation of Hyper, Hypo and Neomorphic Mutations

Project description:Systematic Pan-cancer Functional Inference and Validation of Hyper, Hypo and Neomorphic Mutations

| PRJNA976220 | ENA

The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 17 h

Project description:To delineate cellular processes involved in NiV FG combination and mutants with different fusiogenecity, we have employed whole genome microarray expression profiling as a discovery platform to identify the upregulated and downregulated genes. Total RNA was collected from transfected cells and untransfected controls. Cy3 labeled cRNA is used for hybridization platform Agilent Human 4 x 44K gene expression array.

2025-02-01 | GSE166701 | GEO

The effect of fusiogenicity on transcription profile: Transcription profiles of hyper-fusiogenic and hypo-fusiogenic mutants were tested on HEK293T cells via transfection at 21 h

2025-02-01 | GSE166700 | GEO

Identification of novel hyper- or hypo-methylated CpG sites and genes associated with anthracycline-induced cardiomyopathy

Project description:Identification of novel hyper- or hypo-methylated CpG sites and genes associated with anthracycline-induced cardiomyopathy

| PRJNA930712 | ENA

Divergent transcriptomic profiles in depressed individuals with hyper- and hypophagia implicating inflammatory status

Project description:We used bulk RNAseq to examine differences in blood transcriptomic profiles between depressed individuals with hyperphagia (MDD-hyper) compared to depressed individuals with hypophagia (MDD-hypo) at rest before and after the stress task, controlling for repeated measures.

2024-11-13 | GSE231347 | GEO

Transcription profiling of human dorsal forebrain organoids treated with Hyper-IL-6

Project description:Transcription profiling of human dorsal forebrain organoids treated with Hyper-IL-6

| PRJEB60352 | ENA

ChIP-Seq Analysis of Eed hypo/wt

Project description:ChIP-Seq Analysis of Eed hypo/wt

| PRJEB12273 | ENA