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MYO10-filopodia support basement membranes at pre-invasive tumor boundaries

ABSTRACT: MYO10-filopodia support basement membranes at pre-invasive tumor boundaries

PROVIDER: PRJNA702170 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13724734_1.fastq.gz	Fastqsanger.gz
	SRR13724734_2.fastq.gz	Fastqsanger.gz
	SRR13724735_1.fastq.gz	Fastqsanger.gz
	SRR13724735_2.fastq.gz	Fastqsanger.gz
	SRR13724736_1.fastq.gz	Fastqsanger.gz

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MYO10-filopodia support basement membranes at pre-invasive tumor boundaries

Project description:Myosin-X -silenced and control-silenced subcutaneous breast tumor xenografts in female NOD.scid mice

2022-09-27 | GSE166898 | GEO

Recombinant Spider Silk Membranes Promote Human Renal Epithelial Differentiation and Function

Project description:Porous membranes are frequently used as supports of cell monolayers in functional studies of epithelial and endothelial barriers. However, conventional polymer-based membranes such as those made of polycarbonate do not mimic the structural and biochemical properties of native basement membranes, which may limit cellular differentiation and function. Here, we use a nanofibrillar membrane made of recombinant spider silk functionalized with the integrin-binding RGD motif of fibronectin and coated with human kidney-specific laminin-521 (LN/FN-silk) as a novel substrate for renal epithelial monolayer cultures. Cellular morphology, mRNA expression, barrier properties and transporter activity were assessed using scanning and transmission electron microscopy, RNA-sequencing, lucifer yellow permeability assays, immunofluorescence staining and fluorescent probe transport assays. Immortalized renal proximal tubular epithelial cell (RPTEC/TERT1) monolayers cultured on LN/FN-silk membranes and in co-culture with human fibroblasts (fHDF/TERT166) exhibited cuboidal morphology, reduced cell death and maintained barrier integrity with tight junction expression compared to conventional polymer-based transwell membranes. In contrast to the LN/FN-silk membranes, the polycarbonate membranes released the endocrine disrupting toxicant bisphenol A, triggering estrogen-mediated signalling. Furthermore, cells on LN/FN-silk membranes showed enhanced directional anion and cation transport, compared to cells cultured on conventional membranes. In summary, the results indicate that LN/FN-silk membranes improve differentiation and functional maintenance of renal epithelial cell cultures, thereby allowing preservation of renal epithelial transport functions.

2026-01-22 | GSE307014 | GEO

Expression data from fetal membranes

Project description:Genome wide expression profiling was used to identify signifnificantly changed genes in fetal membranes after GBS treatment Genome-wide expression profiles in GBS-inoculated human extraplacental membranes in vitro were used to identify transcriptomic pathways altered by GBS . Using bioinformatics analysis of these expression profiles we aimed to test our hypothesis that GBS activates pathways related to inflammation and premature birth in human extraplacental membranes.

2018-01-18 | GSE96557 | GEO

Reverse Osmosis Membranes Fractions

Project description:Reverse Osmosis Membranes Fractions Raw sequence reads

| PRJNA629347 | ENA

Basement membranes at a glance.

Project description: Not available

| S-EPMC12450471 | biostudies-literature

Basement membranes and autoimmune diseases.

Project description: Not available

| S-EPMC5290253 | biostudies-literature

Gene expression profile of a C. elegans Anchor cell invading through Basement Membrane

Project description:Cell invasion through basement membrane is crucial for development and immune cell trafficking, and is misregulated in many human diseases, such as cancer. Understanding the gene expression profile of invading cells is challening, because cell invasion events are often stochastic and rare. Thus, it has been difficult to isolate cells during the act of basement membrane invasion. Here, we have taken advantage of the stereotyped timing of anchor cell invasion in C. elegans, along with synchronized animal culturing, Fluorescence activated cell sorting (FACS), and RNA-Seq and identified the gene expression profile of an invasive cell at the time of basement membrane breaching.

2023-04-13 | GSE228962 | GEO

Transcriptomic analysis delineates preterm prelabor rupture of membranes from preterm labor in preterm fetal membranes

Project description:We compared fetal membrane tissue from preterm labor deliveries to fetal tissue from preterm labor with preterm prelabor rupture of membranes (PPROM) deliveries to further explore the concept that spontaneous preterm birth can result from the initializing of two separate but overlapping pathological events. Chorioamnion, separated into amnion and chorion, was collected from gestationally age-matched cases and controls within 15 minutes of birth, and analyzed using RNA sequencing. In our study, transcriptome analysis of preterm fetal membranes revealed distinct differentially expressed genes for PPROM, separate from preterm labor. This study is the first to report transcriptome data that reflects the individual pathophysiology of amnion and chorion tissue from PPROM deliveries.

2024-03-13 | GSE243831 | GEO

Transcriptomic analysis delineates preterm prelabor rupture of membranes from preterm labor in preterm fetal membranes

Project description:Transcriptomic analysis delineates preterm prelabor rupture of membranes from preterm labor in preterm fetal membranes

| PRJNA1020124 | ENA

Long-distance association of topological boundaries through nuclear condensates (Hi-C)

Project description:Chromatin is partitioned into distinct topological domains in an activity-dependent manner, with topological boundaries limiting the interaction between adjacent domains. Recent studies support the concept that several well-established nuclear compartments are assembled as ribonucleoprotein condensates. Here we ask whether the physical processes driving the assembly of the nuclear condensates play any role in three-dimensional chromatin architecture. We report that the insulation of approximately 20% of topological boundaries in human embryonic stem cells is substantially weakened following brief treatment with 1,6-hexanediol, a chemical known to disrupt several nuclear condensates. The disrupted boundaries are characterized by a high level of transcription, striking spatial clustering, and the augmented presence of transcription units widely expressed in diverse cell types. These topological boundary regions tend to be spatially associated, even inter-chromosomally, and segregate with nuclear speckles. These observations reveal a previously unappreciated mode of genome organization mediated by conserved boundary elements harboring widely-expressed transcription units and associated transcriptional condensates.

2022-06-15 | GSE195565 | GEO

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