Project description:The present project deals with bark beetle gut total proteome from callow and black bark beetle, Ips typographus. The study aims to identify life stage-specific expression of gut proteins in bark beetles and their functional relevance.
Project description:Seasonal nitrogen (N) storage and reuse is important to the N-use efficiency of temperate deciduous trees. In poplar, bark storage proteins (BSPs) accumulate in protein storage vacuoles of the bark parenchyma and xylem ray cells in the fall. During spring growth, N from stored BSPs is remobilized and utilized by growing shoots. The goal of this study is to investigate global gene expression changes in the bark during BSP remobilization and shoot regrowth under long-day conditions.
Project description:The periderm of trees produces cork cells, whose cell walls are modified with suberin. We compared the transcriptome of outer bark (cork) vs inner bark (control containing secondary phloem and vacular meristem) to infer genes related to suberim metabolism.
Project description:Seasonal nitrogen (N) storage and reuse is important to the N-use efficiency of temperate deciduous trees. In poplar, bark storage proteins (BSPs) accumulate in protein storage vacuoles of the bark parenchyma and xylem ray cells in the fall. During spring growth, N from stored BSPs is remobilized and utilized by growing shoots. The goal of this study is to investigate global gene expression changes in the bark during BSP remobilization and shoot regrowth under long-day conditions. Long-day (LD) grown poplar (Populus trichocarpa, Nisqually-1) plants were transferred to short-day (SD) for 8 weeks at 20°C followed by an addition 12 weeks of SD at 10°C (day) and 4°C (night). Following this treatment plants were then moved to LD and 20°C for 3 weeks for regrowth. Bark samples were collected from plants released from dormancy just prior to transfer to LD and at weekly intervals for 3 weeks after exposure to LD at 20°C.
Project description:<p>This study applied untargeted metabolomics to investigate the metabolite diversity of <em>Acacia mearnsii</em> (Black Wattle) bark extracts using different solvents. Additionally, it aimed to characterize the proanthocyanidin profile of Black Wattle bark cultivated in southern Brazil. The findings demonstrated that solvent choice significantly influences metabolite extraction and composition. The most pronounced differences were observed between water-based and organic solvent extractions, with organic solvents proving more effective. Among them, 50% and 70% acetone, as well as 70% methanol, yielded the highest metabolite abundances. Annotated compounds included organic acids, phenolic compounds, and coumarins, with several phenolics, such as naringenin, and liquiritigenin, annotated in Black Wattle bark for the first time. These results highlight the richness of its extracts in bioactive compounds, demonstrating a chemical complexity that extends beyond proanthocyanidins and may influence their biological properties. The extracts contained a diverse range of proanthocyanidins, up to pentamers. Monomers such as (epi)catechin and (epi)gallocatechin were identified, while the oligomers were predominantly prorobinetinidins, consisting of catechin or gallocatechin as starter units and robinetinidol or fisetinidol as extender units. The presence of a wide range of bioactive compounds underscores the potential of Black Wattle bark extracts for applications in the food and feed industries.</p>
