Project description:To study the spatial localisations of the cell populations in an early haematopoietic tissue and lymphoid organs critical for T and B cell development, we profiled fetal liver, thymus and spleen from 3 donors at 18 PCW with sequencing-based spatial transcriptomics (10x Genomics Visium).
Project description:These samples are part of a study investigating cancer cell plasticity in colorectal cancer metastasis. Spatial transcriptomics was performed using 10x Genomics Visium on colorectal cancer liver metastatic patient samples.
Project description:These data were used in the spatial transcriptomics analysis of the article titled \\"Single-Cell and Spatial Transcriptomics Analysis of Human Adrenal Aging\\".
Project description:Spatial organization of different cell types within prenatal skin across various anatomical sites is not well understood. To address this, here we have generated spatial transcriptomics data from prenatal facial and abdominal skin obtained from a donor at 10 post conception weeks. This in combination with our prenatal skin scRNA-seq dataset has helped us map the location of various identified cell types.
Project description:These samples are part of a study to provide a spatially resolved single-cell multiomics map of human trophoblast differentiation in early pregnancy. Here we profiled with 10x Visium Spatial transcriptomics of the entire maternal-fetal interface including the myometrium, allowing us to resolve the full trajectory of trophoblast differentiation.
Project description:A comprehensive understanding of the fetal liver niche during development offers a lens into a unique natural multifunctional multicellular environment. Here we use a spatial transcriptomic and histologic analysis approach to identify and histologically confirm RNA-based markers in human fetal liver tissue within a key developmental window in which the liver is a major site of hematopoiesis, just prior to the increased growth phase of the final trimester. Within this window, the fetal liver niche encompasses the unique coexistence of cells of both endodermal and mesenchymal origin with the unique environment fostering hematopoietic cell development as well as hepatocyte function. Single-cell resolution spatial imaging reveals epithelial, hematopoietic, endothelial, and stromal cell populations in shared cellular microenvironments. Here, our single-cell spatial transcriptomic and imaging approach enables, captures, and confirms the complex phenomena of ductal plate expression and CXCL12 homing to the hematopoietic stem cell niche (HSC) in the liver. An RNA-based understanding of the functional diversity and in situ spatial organization of the fetal liver niche is critical for adequate validation and authentication in recapitulating and re-engineering these regenerative environments in vitro.