Project description:Decreased mineral density is a risk factor for skeletal pathologies including bone metastasis, the leading cause of mortality in patients with advanced breast cancer, but the underlying mechanisms are poorly understood. While reduced mineral density can drive pathological bone remodeling via direct effects on select cell types, indirect effects due to broad changes of the microenvironment may be similarly important. However, how bone mineral content affects microenvironmental heterogeneity remains to be elucidated. Here, we leverage decellularized bone matrices with varied mineral content in combination with single-cell RNA-sequencing to study how reduced bone mineral content affects microenvironmental complexity and tumor growth. We performed single-cell RNA sequencing on implanted decellularized bovine bone scaffolds in which the mineral was either maintained at physiological levels or removed to simulate scenarios of impaired bone mineralization as, for example, present during aging. Using this approach, we explored the heterogeneous stromal response to varied bone mineral content in both an immunocompromised and immunocompetent, syngeneic mouse model in the presence and absence of cancer cells.

2024-03-04 | GSE256109 | GEO

Temporal transcriptome analysis of Bacillus subtilis NDmed in the submerged biofilm model

Project description:Investigation of the kinetics of whole genome gene expression level changes in Bacillus subtilis NDmed strain during formation of submerged biofilm and pellicle. The Bacillus subtilis NDmed strain analyzed in this study is able to form thick and highly structured submerged biofilms as described in Bridier et al., (2011) The Spatial Architecture of Bacillus subtilis Biofilms Deciphered Using a Surface-Associated Model and In Situ Imaging. PLoS ONE 6(1):e16177.

2021-12-09 | GSE190460 | GEO

Submerged Analog Habitat Environment

Project description:Submerged Analog Habitat Environment Raw sequence reads

| PRJNA639658 | ENA

Project description:Metagenomic analysis of microorganisms from Movile Cave - Romania

| PRJNA673084 | ENA

Mineral spring water biofilms

Project description:Mineral spring water biofilms Metagenome

| PRJNA1085763 | ENA

Toscana virus – Romania

Project description:Emergence of Toscana virus in Romania: meningoencephalitis case series in 2017-18

| PRJEB35101 | ENA